ArticleThe Journal of biological chemistry2012
Kcnh1 voltage-gated potassium channels are essential for early zebrafish development.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Intellectual Disability and Potassium Channelopathies: A Systematic Review.Frontiers in genetics · 2020Pooled it
- Evolutionary Analysis of Vertebrate KCNH Voltage-Gated Potassium Channels and Spatial Expression ofJournal of developmental biology · 2026Article
- Amitriptyline inhibits EAG1 channels by binding to their PAS domains and exerts EAG1-dependent anti-tumorigenic effects.Cancer gene therapy · 2026Article
- Evolutionary analysis of vertebrate KCNH voltage-gated potassium channels and their expression in zebrafish embryos.bioRxiv : the preprint server for biology · 2026Article
- Multi-endpoint assessment of tunnel wash water and tyre-particle leachate in zebrafish larvae.Toxicology reports · 2025Article
- Intergenerational effects of early life protein restriction on adipose tissue development as revealed by sheep transcriptomic analyses.Scientific reports · 2025Article
- Towards Zebrafish Models of CNS Channelopathies.International journal of molecular sciences · 2022Review
- The Kv10.1 Channel: A Promising Target in Cancer.International journal of molecular sciences · 2022Review
- The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.Stem cells international · 2022Article
- Effective processing and evaluation of chemical imaging data with respect to morphological features of the zebrafish embryo.Analytical and bioanalytical chemistry · 2021Article
- Article
- Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is More Stressful to the SilversideFrontiers in genetics · 2018Article
- Cullin 7 mediates proteasomal and lysosomal degradations of rat Eag1 potassium channels.Scientific reports · 2017Article
- Eag1 KOxidative medicine and cellular longevity · 2017Review
- De novo KCNH1 mutations in four patients with syndromic developmental delay, hypotonia and seizures.Journal of human genetics · 2016Review
- KV 10.1 opposes activity-dependent increase in Ca²⁺ influx into the presynaptic terminal of the parallel fibre-Purkinje cell synapse.The Journal of physiology · 2015Article
- Oxidative modulation of voltage-gated potassium channels.Antioxidants & redox signaling · 2014Review
- Behavioural and functional characterization of Kv10.1 (Eag1) knockout mice.Human molecular genetics · 2013Article
- Cysteines control the N- and C-linker-dependent gating of KCNH1 potassium channels.Biochimica et biophysica acta · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Kcnh1 gene encodes a voltage-gated potassium channel highly expressed in neurons and involved in tumor cell proliferation, yet its physiological roles remain unclear. We have used the zebrafish as a model to analyze Kcnh1 function in vitro and in vivo. We found that the kcnh1 gene is duplicated in teleost fish (i.e. kcnh1a and kcnh1b) and that both genes are maternally expressed during early development. In adult zebrafish, kcnh1a and kcnh1b have distinct expression patterns but share expression in brain and testis. Heterologous expression of both genes in Xenopus oocytes revealed a strong conservation of characteristic functional properties between human and fish channels, including a unique sensitivity to intracellular Ca(2+)/calmodulin and modulation of voltage-dependent gating by extracellular Mg(2+). Using a morpholino antisense approach, we demonstrate a strong kcnh1 loss-of-function phenotype in developing zebrafish, characterized by growth retardation, delayed hindbrain formation, and embryonic lethality. This late phenotype was preceded by transcriptional up-regulation of known cell-cycle inhibitors (p21, p27, cdh2) and down-regulation of pro-proliferative factors, including cyclin D1, at 70% epiboly. These results reveal an unanticipated basic activity of kcnh1 that is crucial for early embryonic development and patterning.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.