Trial reportThe Journal of biological chemistry2012
Identification of a novel role for dematin in regulating red cell membrane function by modulating spectrin-actin interaction.
Trial report in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Erythrocytes membrane fluidity changes induced by adenylyl cyclase cascade activation: study using fluorescence recovery after photobleaching.European biophysics journal : EBJ · 2024Article
- Proteomes of Micro- and Nanosized Carriers Engineered from Red Blood Cells.Journal of proteome research · 2023Article
- The Role of Liquid-Liquid Phase Separation in Actin Polymerization.International journal of molecular sciences · 2023Review
- A preliminary study of phosphodiesterases and adenylyl cyclase signaling pathway on red blood cell deformability of sickle cell patients.Frontiers in physiology · 2023Article
- Self-construction of actin networks through phase separation-induced abLIM1 condensates.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Mechanical behavior of actin and spectrin subjected to high strain rate: A molecular dynamics simulation study.Computational and structural biotechnology journal · 2021Article
- An exported kinase family mediates species-specific erythrocyte remodelling and virulence in human malaria.Nature microbiology · 2020Article
- DNA methylation changes in infants between 6 and 52 weeks.Scientific reports · 2019Article
- Hypermethylation of DMTN promotes the metastasis of colorectal cancer cells by regulating the actin cytoskeleton through Rac1 signaling activation.Journal of experimental & clinical cancer research : CR · 2018Article
- Non-muscle myosin II drives vesicle loss during human reticulocyte maturation.Haematologica · 2018Article
- abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing.Cell discovery · 2018Article
- Defective erythropoiesis caused by mutations of the thyroid hormone receptor α gene.PLoS genetics · 2017Article
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- Quantitative phospho-proteomics reveals the Plasmodium merozoite triggers pre-invasion host kinase modification of the red cell cytoskeleton.Scientific reports · 2016Article
- Proteome analysis of the triton-insoluble erythrocyte membrane skeleton.Journal of proteomics · 2015Article
- Inositol Hexakisphosphate Kinase-3 Regulates the Morphology and Synapse Formation of Cerebellar Purkinje Cells via Spectrin/Adducin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- cAMP-Signalling Regulates Gametocyte-Infected Erythrocyte Deformability Required for Malaria Parasite Transmission.PLoS pathogens · 2015Article
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- Spectrin and phospholipids - the current picture of their fascinating interplay.Cellular & molecular biology letters · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The membrane skeleton plays a central role in maintaining the elasticity and stability of the erythrocyte membrane, two biophysical features critical for optimal functioning and survival of red cells. Many constituent proteins of the membrane skeleton are phosphorylated by various kinases, and phosphorylation of β-spectrin by casein kinase and of protein 4.1R by PKC has been documented to modulate erythrocyte membrane mechanical stability. In this study, we show that activation of endogenous PKA by cAMP decreases membrane mechanical stability and that this effect is mediated primarily by phosphorylation of dematin. Co-sedimentation assay showed that dematin facilitated interaction between spectrin and F-actin, and phosphorylation of dematin by PKA markedly diminished this activity. Quartz crystal microbalance measurement revealed that purified dematin specifically bound the tail region of the spectrin dimer in a saturable manner with a submicromolar affinity. Pulldown assay using recombinant spectrin fragments showed that dematin, but not phospho-dematin, bound to the tail region of the spectrin dimer. These findings imply that dematin contributes to the maintenance of erythrocyte membrane mechanical stability by facilitating spectrin-actin interaction and that phosphorylation of dematin by PKA can modulate these effects. In this study, we have uncovered a novel functional role for dematin in regulating erythrocyte membrane function.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.