ArticleProceedings of the National Academy of Sciences of the United States of America2012
Structural basis for entropy-driven cellulose binding by a type-A cellulose-binding module (CBM) and bacterial expansin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 190 citations in OpenAlex.
- O-Glycosylated expansinScience advances · 2026Article
- Functional characterization of a dockerin-containing expansin-like protein from the anaerobic fungus Neocallimastix californiae.Biotechnology for biofuels and bioproducts · 2026Article
- Influence of Structural Features of Peptides on Their Affinity to Cotton Linters Paper.ACS omega · 2026Article
- Uncovering Sequence and Structural Characteristics of Fungal Expansin-Related Proteins With Potential to Drive Substrate Targeting.Proteins · 2026Article
- Molecular Biomarkers in IgE Immunoassays Used for Grass Pollen Allergy Diagnosis in European Clinical Settings.International journal of molecular sciences · 2026Review
- The impact of rumen and hindgut microbiomes on the persistent productivity of long-lived dairy cows.Microbiome · 2026Article
- Multi-omics reveals growth regulatory mechanisms induced by exogenous TgSWO expansin-like protein in strawberry.Frontiers in plant science · 2026Article
- Mining expansin-like proteins from rumen microbiota and functional characterization of two anaerobic fungal expansin-like proteins.Journal of animal science and biotechnology · 2025Article
- The Bacillus subtilis expansin BsEXLX1 enhances cellobiohydrolase I activity on sugar beet pulp as shown by QCM-D.Biotechnology letters · 2025Article
- A High-Throughput Investigation of the Binding Specificity of Carbohydrate-Binding Modules for Synthetic and Natural Polymers.ChemSusChem · 2025Article
- Molecular Moieties Having Pyrene and Carboxylic Acid for the Hydrolysis of Cellulose.ACS omega · 2025Article
- Expansins in Salt and Drought Stress Adaptation: From Genome-Wide Identification to Functional Characterisation in Crops.Plants (Basel, Switzerland) · 2025Review
- Adsorption Capacity of CBM104s Appended to Different Types of Catalytic Domains.Journal of applied glycoscience · 2025Article
- Aromatic residues in N-terminal domain of archaeal trehalase affect the folding and activity of catalytic domain.Applied microbiology and biotechnology · 2024Article
- Diaminated Cellulose Beads as a Sustainable Support for Industrially Relevant Lipases.ACS sustainable chemistry & engineering · 2024Article
- Structure and growth of plant cell walls.Nature reviews. Molecular cell biology · 2024Review
- Insights into the action of phylogenetically diverse microbial expansins on the structure of cellulose microfibrils.Biotechnology for biofuels and bioproducts · 2024Article
- Transcriptome- and genome-wide systematic identification of expansin gene family and their expression in tuberous root development and stress responses in sweetpotato (Frontiers in plant science · 2024Article
- Genome-Wide Analysis of the Expansin Gene Family inInternational journal of molecular sciences · 2023Article
- Loosenin-Like Proteins fromApplied and environmental microbiology · 2023Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Components of modular cellulases, type-A cellulose-binding modules (CBMs) bind to crystalline cellulose and enhance enzyme effectiveness, but structural details of the interaction are uncertain. We analyzed cellulose binding by EXLX1, a bacterial expansin with ability to loosen plant cell walls and whose domain D2 has type-A CBM characteristics. EXLX1 strongly binds to crystalline cellulose via D2, whereas its affinity for soluble cellooligosaccharides is weak. Calorimetry indicated cellulose binding was largely entropically driven. We solved the crystal structures of EXLX1 complexed with cellulose-like oligosaccharides to find that EXLX1 binds the ligands through hydrophobic interactions of three linearly arranged aromatic residues in D2. The crystal structures revealed a unique form of ligand-mediated dimerization, with the oligosaccharide sandwiched between two D2 domains in opposite polarity. This report clarifies the molecular target of expansin and the specific molecular interactions of a type-A CBM with cellulose.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.