ArticleProceedings of the National Academy of Sciences of the United States of America2012
An insect-specific P450 oxidative decarbonylase for cuticular hydrocarbon biosynthesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 163 papers, 1 of them a synthesis that pooled it.
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Who cites it
163 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Evolution of Cuticular Hydrocarbons in the Hymenoptera: a Meta-Analysis.Journal of chemical ecology · 2015Pooled it
- Cytochrome P450-mediated regulation of pheromone biosynthesis and adult emergence in Monochamus saltuarius (Coleoptera, Cerambycidae), with implications for pest management.Pest management science · 2026Article
- CYP6AB328-mediated spinetoram resistance via N-demethylation in Tuta absoluta: A potential resistance monitoring marker.Pest management science · 2026Article
- De novo synthesis of short-chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys.FEBS open bio · 2026Article
- Lipid metabolism of hepatocyte-like cells supports intestinal tumor growth in Drosophila.Nature communications · 2026Article
- Independent Evolution of Linear and Branched Cuticular Hydrocarbons in Ants: A Hypothesis.Insects · 2026Article
- Cytochrome P450 Genes Mediate High-Temperature Adaptation Under Diverging Humidity Conditions inInternational journal of molecular sciences · 2026Article
- Investigating genetic differentiation between brackish and fresh water collections of the arboviral vector Aedes aegypti.Parasites & vectors · 2026Article
- Complex Roles of Insect Cytochrome P450s in Chemical Adaptation.International journal of biological sciences · 2026Review
- Functional Annotation and Comparative Analysis of Cytochrome P450 Protein Family Genes in Nine Chironomidae Species.Biology · 2025Article
- Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal.Cell genomics · 2025Article
- Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants.Nature communications · 2025Article
- Directed evolution of hydrocarbon-producing enzymes.Biotechnology for biofuels and bioproducts · 2025Review
- De Novo transcriptome assembly of Diaprepes abbreviatus: foundations for functional genomics and targeted pest management.BMC genomics · 2025Article
- Regulatory changes in the fatty acid elongase eloF underlie the evolution of sex-specific pheromone profiles in Drosophila prolongata.BMC biology · 2025Article
- Expression of Elongase- and Desaturase-Encoding Genes Shapes the Cuticular Hydrocarbon Profiles of Honey Bees.Molecular ecology · 2025Article
- Via Air or Rhizosphere: The Phytotoxicity ofPlants (Basel, Switzerland) · 2025Review
- Pathway elucidation and heterologous reconstitution of the long-chain alkane pentadecane biosynthesis from Pogostemon cablin.Plant biotechnology journal · 2025Article
- Genetic Underpinnings of Cuticular Hydrocarbon Biosynthesis in the German Cockroach, Blattella germanica (L.): Progress and Perspectives.Journal of chemical ecology · 2024Review
- Cuticular hydrocarbons promote desiccation resistance by preventing transpiration in Drosophila melanogaster.The Journal of experimental biology · 2024Article
103 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insects use hydrocarbons as cuticular waterproofing agents and as contact pheromones. Although their biosynthesis from fatty acyl precursors is well established, the last step of hydrocarbon biosynthesis from long-chain fatty aldehydes has remained mysterious. We show here that insects use a P450 enzyme of the CYP4G family to oxidatively produce hydrocarbons from aldehydes. Oenocyte-directed RNAi knock-down of Drosophila CYP4G1 or NADPH-cytochrome P450 reductase results in flies deficient in cuticular hydrocarbons, highly susceptible to desiccation, and with reduced viability upon adult emergence. The heterologously expressed enzyme converts C(18)-trideuterated octadecanal to C(17)-trideuterated heptadecane, showing that the insect enzyme is an oxidative decarbonylase that catalyzes the cleavage of long-chain aldehydes to hydrocarbons with the release of carbon dioxide. This process is unlike cyanobacteria that use a nonheme diiron decarbonylase to make alkanes from aldehydes with the release of formate. The unique and highly conserved insect CYP4G enzymes are a key evolutionary innovation that allowed their colonization of land.
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