ArticleProceedings of the National Academy of Sciences of the United States of America2012
Foldon unfolding mediates the interconversion between M(pro)-C monomer and 3D domain-swapped dimer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- An isothermal calorimetry assay for determining steady state kinetic and Ensitrelvir inhibition parameters for SARS-CoV-2 3CL-protease.Scientific reports · 2024Article
- SARS-CoV-2 MACS central science · 2023Article
- Discovery of 2-thiobenzimidazoles as noncovalent inhibitors of SARS-CoV-2 main protease.Bioorganic & medicinal chemistry letters · 2022Article
- Is amyloid fibrillation related to 3D domain swapping for the C-terminal domain of SARS-CoV main protease?International journal of biological macromolecules · 2022Article
- A multi-pronged approach targeting SARS-CoV-2 proteins using ultra-large virtual screening.iScience · 2021Article
- Exposing the distinctive modular behavior of β-strands and α-helices in folded proteins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Multi-Pronged Approach Targeting SARS-CoV-2 Proteins Using Ultra-Large Virtual Screening.ChemRxiv : the preprint server for chemistry · 2020Article
- The protonation state of an evolutionarily conserved histidine modulates domainswapping stability of FoxP1.Scientific reports · 2019Article
- Understanding protein domain-swapping using structure-based models of protein folding.Progress in biophysics and molecular biology · 2017Review
- A Simple Model of Protein Domain Swapping in Crowded Cellular Environments.Biophysical journal · 2016Article
- Domain swapping oligomerization of thermostable c-type cytochrome in E. coli cells.Scientific reports · 2016Article
- Domain-swapped cytochromeChemical science · 2015Article
- Biophysical analysis of the MHR motif in folding and domain swapping of the HIV capsid protein C-terminal domain.Biophysical journal · 2015Article
- Coronaviruses resistant to a 3C-like protease inhibitor are attenuated for replication and pathogenesis, revealing a low genetic barrier but high fitness cost of resistance.Journal of virology · 2014Article
- Dimeric switch of Hakai-truncated monomers during substrate recognition: insights from solution studies and NMR structure.The Journal of biological chemistry · 2014Article
- Mechanism of 3D domain swapping in bovine seminal ribonuclease.The FEBS journal · 2014Article
- Proline substitution of dimer interface β-strand residues as a strategy for the design of functional monomeric proteins.Biophysical journal · 2013Article
- Evidences for the unfolding mechanism of three-dimensional domain swapping.Protein science : a publication of the Protein Society · 2013Article
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6 authors.
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Abstract
The C-terminal domain (M(pro)-C) of SARS-CoV main protease adopts two different fold topologies, a monomer and a 3D domain-swapped dimer. Here, we report that M(pro)-C can reversibly interconvert between these two topological states under physiological conditions. Although the swapped α(1)-helix is fully buried inside the protein hydrophobic core, the interconversion of M(pro)-C is carried out without the hydrophobic core being exposed to solvent. The 3D domain swapping of M(pro)-C is activated by an order-to-disorder transition of its C-terminal α(5)-helix foldon. Unfolding of this foldon promotes self-association of M(pro)-C monomers and functions to mediate the 3D domain swapping, without which M(pro)-C can no longer form the domain-swapped dimer. Taken together, we propose that there exists a special dimeric intermediate enabling the protein core to unpack and the α(1)-helices to swap in a hydrophobic environment, which minimizes the energy cost of the 3D domain-swapping process.
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