ArticleBMC biotechnology2012
Enhanced itaconic acid production in Aspergillus niger using genetic modification and medium optimization.
Article in BMC biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 73 citations in OpenAlex.
- Mechanistic and structural insights into the itaconate-producingPNAS nexus · 2025Article
- Conversion of Polyethylenes into Fungal Secondary Metabolites.Angewandte Chemie (International ed. in English) · 2023Article
- Review
- Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications.FEMS microbiology letters · 2020Review
- Deletion analysis of the itaconic acid biosynthesis gene cluster components in Aspergillus pseudoterreus ATCC32359.Applied microbiology and biotechnology · 2020Article
- Metabolic engineering of an industrial3 Biotech · 2020Article
- The crystal structure of mouse IRG1 suggests that cis-aconitate decarboxylase has an open and closed conformation.PloS one · 2020Article
- Disruption of a putative mitochondrial oxaloacetate shuttle protein in Aspergillus carbonarius results in secretion of malic acid at the expense of citric acid production.BMC biotechnology · 2019Article
- Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers.Polymers · 2019Review
- A review on commercial-scale high-value products that can be produced alongside cellulosic ethanol.Biotechnology for biofuels · 2019Review
- Itaconic acid degradation inFungal biology and biotechnology · 2019Article
- Identification of novel citramalate biosynthesis pathways inFungal biology and biotechnology · 2019Article
- Metabolic engineering with ATP-citrate lyase and nitrogen source supplementation improves itaconic acid production inBiotechnology for biofuels · 2019Article
- Itaconic acid production in microorganisms.Biotechnology letters · 2018Review
- World market and biotechnological production of itaconic acid.3 Biotech · 2018Review
- PApplied and environmental microbiology · 2017Article
- Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical.Journal of industrial microbiology & biotechnology · 2017Review
- Efficient itaconic acid production from glycerol withBiotechnology for biofuels · 2017Article
- Rewiring a secondary metabolite pathway towards itaconic acid production in Aspergillus niger.Microbial cell factories · 2016Article
- Ustilago maydis produces itaconic acid via the unusual intermediate trans-aconitate.Microbial biotechnology · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAspergillus niger was selected as a host for producing itaconic acid due to its versatile and tolerant character in various growth environments, and its extremely high capacity of accumulating the precursor of itaconic acid: citric acid. Expressing the CAD gene from Aspergillus terreus opened the metabolic pathway towards itaconic acid in A. niger. In order to increase the production level, we continued by modifying its genome and optimizing cultivation media.
resultsBased on the results of previous transcriptomics studies and research from other groups, two genes : gpdA encoding the glyceraldehyde -3-dehydrogenase (GPD) and hbd1 encoding a flavohemoglobin domain (HBD) were overexpressed in A. niger. Besides, new media were designed based on a reference medium for A. terreus. To analyze large numbers of cultures, we developed an approach for screening both fungal transformants and various media in 96-well micro-titer plates. The hbd1 transformants (HBD 2.2/2.5) did not improve itaconic acid titer while the gpdA transformant (GPD 4.3) decreased the itaconic acid production. Using 20 different media, copper was discovered to have a positive influence on itaconic acid production. Effects observed in the micro-titer plate screening were confirmed in controlled batch fermentation.
conclusionsThe performance of gpdA and hbd1 transformants was found not to be beneficial for itaconic acid production using the tested cultivation conditions. Medium optimization showed that, copper was positively correlated with improved itaconic acid production. Interestingly, the optimal conditions for itaconic acid clearly differ from conditions optimal for citric- and oxalic acid production.
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