ArticleJournal of molecular biology2012
Characterization of transport proteins for aromatic compounds derived from lignin: benzoate derivative binding proteins.
Article in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- The oleaginous yeast Cutaneotrichosporon oleaginosum modifies corn stover alkali lignin.Scientific reports · 2026Article
- Genetically encoded biosensor enabled mining, characterisation and engineering of aromatic acid MFS transporters.Journal of biological engineering · 2025Article
- Uptake system of lignin-derived aromatic acids in promising Sphingomonadaceae strains for lignin valorization through biological funneling.Scientific reports · 2025Article
- Discovery of periplasmic solute binding proteins with specificity for ketone bodies: β-hydroxybutyrate binding proteins.Protein science : a publication of the Protein Society · 2024Article
- Proteogenomic Characterization ofMicroorganisms · 2024Article
- Genome-Wide Identification of the ABC Gene Family and Its Expression in Response to the Wood Degradation of Poplar inJournal of fungi (Basel, Switzerland) · 2024Article
- A Novel Gene Cluster Is Involved in the Degradation of Lignin-Derived Monoaromatics in Thermus oshimai JL-2.Applied and environmental microbiology · 2021Article
- Comparative Proteomics ofMicroorganisms · 2020Article
- Passive membrane transport of lignin-related compounds.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database.Applied microbiology and biotechnology · 2019Review
- Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators.Nature communications · 2018Article
- A novel signal transduction protein: Combination of solute binding and tandem PAS-like sensor domains in one polypeptide chain.Protein science : a publication of the Protein Society · 2017Article
- Transport capabilities of environmental Pseudomonads for sulfur compounds.Protein science : a publication of the Protein Society · 2017Article
- How Aromatic Compounds Block DNA Binding of HcaR Catabolite Regulator.The Journal of biological chemistry · 2016Article
- Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Article
- Evidence supporting dissimilatory and assimilatory lignin degradation in Enterobacter lignolyticus SCF1.Frontiers in microbiology · 2013Article
- Modeling themSystemsArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In vitro growth experiments have demonstrated that aromatic compounds derived from lignin can be metabolized and represent a major carbon resource for many soil bacteria. However, the proteins that mediate the movement of these metabolites across the cell membrane have not been thoroughly characterized. To address this deficiency, we used a library representative of lignin degradation products and a thermal stability screen to determine ligand specificity for a set of solute-binding proteins (SBPs) from ATP-binding cassette (ABC) transporters. The ligand mapping process identified a set of proteins from Alphaproteobacteria that recognize various benzoate derivatives. Seven high-resolution crystal structures of these proteins in complex with four different aromatic compounds were obtained. The protein-ligand complexes provide details of molecular recognition that can be used to infer binding specificity. This structure-function characterization provides new insight for the biological roles of these ABC transporters and their SBPs, which had been previously annotated as branched-chain amino-acid-binding proteins. The knowledge derived from the crystal structures provides a foundation for development of sequence-based methods to predict the ligand specificity of other uncharacterized transporters. These results also demonstrate that Alphaproteobacteria possess a diverse set of transport capabilities for lignin-derived compounds. Characterization of this new class of transporters improves genomic annotation projects and provides insight into the metabolic potential of soil bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.