ArticleEnvironmental microbiology2012
Tagaturonate-fructuronate epimerase UxaE, a novel enzyme in the hexuronate catabolic network in Thermotoga maritima.
Article in Environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Metabolic engineering for microbial production of sugar acids.BMC biotechnology · 2025Review
- The use of isomerases and epimerases for the production of the functional sugars mannose, allulose and tagatose from Fructose.World journal of microbiology & biotechnology · 2025Review
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- Non-Native Site-Selective Enzyme Catalysis.Chemical reviews · 2023Review
- E. coli allantoinase is activated by the downstream metabolic enzyme, glycerate kinase, and stabilizes the putative allantoin transporter by direct binding.Scientific reports · 2023Article
- Bacterial Communities in Lanna Fermented Soybeans from Three Different Ethnolinguistic Groups in Northern Thailand.Microorganisms · 2023Article
- A thousand metagenome-assembled genomes ofFrontiers in cellular and infection microbiology · 2022Article
- Identification of a transcription factor, PunR, that regulates the purine and purine nucleoside transporter punC in E. coli.Communications biology · 2021Article
- Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme ThermophilemSystems · 2021Article
- Transcription factor allosteric regulation through substrate coordination to zinc.NAR genomics and bioinformatics · 2021Article
- Genes for degradation and utilization of uronic acid-containing polysaccharides of a marine bacteriumPeerJ · 2021Article
- Application-Oriented Marine Isomerases in Biocatalysis.Marine drugs · 2020Review
- Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases.Scientific reports · 2020Article
- Biological pincer complexes.ChemCatChem · 2020Article
- Microbial hexuronate catabolism in biotechnology.AMB Express · 2019Review
- How Bioinformatic Tools Guide Experiments To Resolve the Chaos of Apparently Unlimited Metabolic Variation.Journal of bacteriology · 2019Article
- Novel Metabolic Pathways and Regulons for Hexuronate Utilization in Proteobacteria.Journal of bacteriology · 2019Article
- A Novel D-Galacturonate Fermentation Pathway inFrontiers in microbiology · 2019Article
- Structure-based prediction and identification of 4-epimerization activity of phosphate sugars in class II aldolases.Scientific reports · 2017Article
- Sugar Metabolism of the First Thermophilic PlanctomyceteFrontiers in microbiology · 2017Article
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5 authors.
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Abstract
Thermotoga maritima is a marine hyperthermophilic microorganism that degrades a wide range of simple and complex carbohydrates including pectin and produces fermentative hydrogen at high yield. Galacturonate and glucuronate, two abundant hexuronic acids in pectin and xylan, respectively, are catabolized via committed metabolic pathways to supply carbon and energy for a variety of microorganisms. By a combination of bioinformatics and experimental techniques we identified a novel enzyme family (named UxaE) catalysing a previously unknown reaction in the hexuronic acid catabolic pathway, epimerization of tagaturonate to fructuronate. The enzymatic activity of the purified recombinant tagaturonate epimerase from T. maritima was directly confirmed and kinetically characterized. Its function was also confirmed by genetic complementation of the growth of the Escherichia coli uxaB knockout mutant strain on galacturonate. An inferred novel galacturonate to mannonate catabolic pathway in T. maritima was reconstituted in vitro using a mixture of recombinant purified enzymes UxaE, UxaC and UxuB. Members of the newly identified UxaE family were identified in ~50 phylogenetically diverse heterotrophic bacteria from aquatic and soil environments. The genomic context of respective genes and reconstruction of associated pathways suggest that UxaE enzymatic and biological function remains conserved in all of these species.
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