ArticleJournal of the American Chemical Society2012
Rational design of allosteric inhibitors and activators using the population-shift model: in vitro validation and application to an artificial biosensor.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- DNA-functionalized artificial mechanoreceptor for de novo force-responsive signaling.Nature chemical biology · 2024Article
- Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transduction.Nature communications · 2023Article
- Functional advantages of building nanosystems using multiple molecular components.Nature chemistry · 2023Article
- Thermo-Programmed Synthetic DNA-Based Receptors.ACS nano · 2023Article
- Programmable self-regulated molecular buffers for precise sustained drug delivery.Nature communications · 2022Article
- Programmable allosteric DNA regulations for molecular networks and nanomachines.Science advances · 2022Article
- Stoichiometric approach to quantitative analysis of biomolecules: the case of nucleic acids.Analytical and bioanalytical chemistry · 2022Article
- Expanding detection windows for discriminating single nucleotide variants using rationally designed DNA equalizer probes.Nature communications · 2020Article
- Cooperative Heteroligand Interaction with G-Quadruplexes Shows Evidence of Allosteric Binding.Biochemistry · 2020Article
- Computational Design of an Allosteric Antibody Switch by Deletion and Rescue of a Complex Structural Constellation.ACS central science · 2020Article
- Single-step multivalent capture assay for nucleic acid detection with dual-affinity regulation using mutation inhibition and allosteric activation.Chemical science · 2019Article
- No Structure-Switching Required: A Generalizable Exonuclease-Mediated Aptamer-Based Assay for Small-Molecule Detection.Journal of the American Chemical Society · 2018Article
- Complementary oligonucleotides regulate induced fit ligand binding in duplexed aptamers.Chemical science · 2017Article
- Allosteric DNA nanoswitches for controlled release of a molecular cargo triggered by biological inputs.Chemical science · 2017Article
- Using Nature's "Tricks" To Rationally Tune the Binding Properties of Biomolecular Receptors.Accounts of chemical research · 2016Review
- Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure.Chemical science · 2016Article
- Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays.Chemical science · 2015Article
- Intrinsic disorder as a generalizable strategy for the rational design of highly responsive, allosterically cooperative receptors.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Rationally manipulating aptamer binding affinities in a stem-loop molecular beacon.Bioconjugate chemistry · 2014Article
- Using the population-shift mechanism to rationally introduce "Hill-type" cooperativity into a normally non-cooperative receptor.Angewandte Chemie (International ed. in English) · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The population-shift mechanism can be used for rational re-engineering of structure-switching biosensors to enable their allosteric inhibition and activation. As a proof-of-principle example of this, we have introduced distal allosteric sites into molecular beacons, which are optical sensors for the detection of specific nucleic acid sequences. The binding of inhibitors and activators to these sites enabled the rational modulation of the sensor's target affinity-and thus its useful dynamic range-over 3 orders of magnitude. The convenience with which this was done suggests that the population-shift mechanism may prove to be a useful method by which allosteric regulation can be introduced into biosensors, "smart" biomaterials, and other artificial biotechnologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.