ArticlePlant physiology2012
The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well-watered conditions but not under water stress.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 120 citations in OpenAlex.
- Optimized protocol for efficient generation, confirmation, transformation, and CRISPR editing of grapevine hairy roots.Journal of experimental botany · 2026Article
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- Biochemical Response and Gene Expression to Water Deficit of Croatian Grapevine Cultivars (Plants (Basel, Switzerland) · 2023Article
- The Rootstock Genotypes Determine Drought Tolerance by Regulating Aquaporin Expression at the Transcript Level and Phytohormone Balance.Plants (Basel, Switzerland) · 2023Article
- Stomatal conductance tracks soil-to-leaf hydraulic conductance in faba bean and maize during soil drying.Plant physiology · 2022Article
- Root pruning improves maize water-use efficiency by root water absorption.Frontiers in plant science · 2022Article
- Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development.BMC plant biology · 2021Article
- Candidate genes and SNPs associated with stomatal conductance under drought stress in Vitis.BMC plant biology · 2021Article
- New Technologies and Strategies for Grapevine Breeding Through Genetic Transformation.Frontiers in plant science · 2021Review
- Molecular Tools for Adapting Viticulture to Climate Change.Frontiers in plant science · 2021Review
- Phosphorylation of plasma membrane aquaporin PIP2;1 in C-terminal affects light-induced stomatal opening in Arabidopsis.Plant signaling & behavior · 2020Article
- Responses of water accumulation and solute metabolism in tomato fruit to water scarcity and implications for main fruit quality variables.Journal of experimental botany · 2020Review
- Physiological andFrontiers in plant science · 2020Article
- Nitrate Uptake and Transport Properties of Two Grapevine Rootstocks With Varying Vigor.Frontiers in plant science · 2020Article
- Does Molecular and Structural Evolution Shape the Speedy Grass Stomata?Frontiers in plant science · 2020Review
- Local root ABA/cytokinin status and aquaporins regulate poplar responses to mild drought stress independently of the ectomycorrhizal fungus Laccaria bicolor.Journal of experimental botany · 2019Article
- Exploiting natural variation and genetic manipulation of stomatal conductance for crop improvement.Current opinion in plant biology · 2019Review
- Article
- Regulation of aquaporins in plants under stress.Biological research · 2018Review
- Ectopically expressing MdPIP1;3, an aquaporin gene, increased fruit size and enhanced drought tolerance of transgenic tomatoes.BMC plant biology · 2017Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;4N is the most expressed PIP2 gene in root. In situ hybridization confirmed root localization in the cortical parenchyma and close to the endodermis. We then constitutively overexpressed VvPIP2;4N in grape 'Brachetto', and in the resulting transgenic plants we analyzed (1) the expression of endogenous and transgenic VvPIP2;4N and of four other aquaporins, (2) whole-plant, root, and leaf ecophysiological parameters, and (3) leaf abscisic acid content. Expression of transgenic VvPIP2;4N inhibited neither the expression of the endogenous gene nor that of other PIP aquaporins in both root and leaf. Under well-watered conditions, transgenic plants showed higher stomatal conductance, gas exchange, and shoot growth. The expression level of VvPIP2;4N (endogenous + transgene) was inversely correlated to root hydraulic resistance. The leaf component of total plant hydraulic resistance was low and unaffected by overexpression of VvPIP2;4N. Upon water stress, the overexpression of VvPIP2;4N induced a surge in leaf abscisic acid content and a decrease in stomatal conductance and leaf gas exchange. Our results show that aquaporin-mediated modifications of root hydraulics play a substantial role in the regulation of water flow in well-watered grapevine plants, while they have a minor role upon drought, probably because other signals, such as abscisic acid, take over the control of water flow.
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