ArticlePlant physiology2012
A set of regioselective O-methyltransferases gives rise to the complex pattern of methoxylated flavones in sweet basil.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- A dihydrochalcone-specific O-methyltransferase from leaf buds of Populus trichocarpa implicated in bud resin formation.Journal of experimental botany · 2025Article
- Engineering an Escherichia coli strain for enhanced production of flavonoids derived from pinocembrin.Microbial cell factories · 2024Article
- Genome-Wide Characterization ofGenes · 2024Article
- Qualitative Profiling, Antioxidant and Antimicrobial Activities of Polar and Nonpolar Basil Extracts.Foods (Basel, Switzerland) · 2024Article
- Systematic Identification and Characterization ofInternational journal of molecular sciences · 2024Article
- Neofunctionalization of anProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Peeling back the layers: Unveiling the biochemistry of health-promoting molecules in orange fruits.Plant physiology · 2023Article
- A multifunctional true caffeoyl coenzyme A O-methyltransferase enzyme participates in the biosynthesis of polymethoxylated flavones in citrus.Plant physiology · 2023Article
- Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.Molecules (Basel, Switzerland) · 2022Review
- Molecular Characterisation of FlavanoneInternational journal of molecular sciences · 2022Article
- Diversification of Chemical Structures of Methoxylated Flavonoids and Genes Encoding Flavonoid-Plants (Basel, Switzerland) · 2022Review
- Biosynthesis and antifungal activity of fungus-induced O-methylated flavonoids in maize.Plant physiology · 2022Article
- Two types of O-methyltransferase are involved in biosynthesis of anticancer methoxylated 4'-deoxyflavones in Scutellaria baicalensis Georgi.Plant biotechnology journal · 2022Article
- Metabolite profiling reveals organ-specific flavone accumulation inPlant direct · 2021Article
- Shaping the biology of citrus: I. Genomic determinants of evolution.The plant genome · 2021Article
- Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss.Scientific reports · 2021Article
- Isolation, separation, identification, and quantification of bioactive methylated flavone regioisomers by UHPLC-MS/MS.Analytical science advances · 2021Article
- Biochemical Characterization of a FlavonoidMolecules (Basel, Switzerland) · 2020Article
- Short-Term Pre-Harvest UV-B Supplement Enhances the Polyphenol Content and Antioxidant Capacity ofPlants (Basel, Switzerland) · 2020Article
- Characterization of a Flavonoid 3'/5'/7-Molecules (Basel, Switzerland) · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymethoxylated flavonoids occur in a number of plant families, including the Lamiaceae. To date, the metabolic pathways giving rise to the diversity of these compounds have not been studied. Analysis of our expressed sequence tag database for four sweet basil (Ocimum basilicum) lines afforded identification of candidate flavonoid O-methyltransferase genes. Recombinant proteins displayed distinct substrate preferences and product specificities that can account for all detected 7-/6-/4'-methylated, 8-unsubstituted flavones. Their biochemical specialization revealed only certain metabolic routes to be highly favorable and therefore likely in vivo. Flavonoid O-methyltransferases catalyzing 4'- and 6-O-methylations shared high identity (approximately 90%), indicating that subtle sequence changes led to functional differentiation. Structure homology modeling suggested the involvement of several amino acid residues in defining the proteins' stringent regioselectivities. The roles of these individual residues were confirmed by site-directed mutagenesis, revealing two discrete mechanisms as a basis for the switch between 6- and 4'-O-methylation of two different substrates. These findings delineate major pathways in a large segment of the flavone metabolic network and provide a foundation for its further elucidation.
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