ArticleThe Plant cell2012
Contamination risks in work with synthetic peptides: flg22 as an example of a pirate in commercial peptide preparations.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Current Understanding of Cell-Surface Immune Receptors for MAMPs and Plant Parasites in the Solanaceae Family.Molecular plant pathology · 2026Review
- Integrated Framework of the Immune-Defense Transcriptional Signatures in the Arabidopsis Shoot Apical Meristem.International journal of molecular sciences · 2020Article
- A Novel Method for Sampling and Long-Term Monitoring of Microbes That Uses Stickers of Plain Paper.Applied and environmental microbiology · 2019Article
- The Ralstonia solanacearum csp22 peptide, but not flagellin-derived peptides, is perceived by plants from the Solanaceae family.Plant biotechnology journal · 2018Article
- AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Detecting the interaction of peptide ligands with plant membrane receptors.Current protocols in plant biology · 2017Article
- Challenges in the identification of microbe-associated molecular patterns in plant and animal innate immunity: a case study with bacterial lipopolysaccharide.Molecular plant pathology · 2016Article
- Identification of a Novel Small Cysteine-Rich Protein in the Fraction from the Biocontrol Fusarium oxysporum Strain CS-20 that Mitigates Fusarium Wilt Symptoms and Triggers Defense Responses in Tomato.Frontiers in plant science · 2015Article
- A conserved peptide pattern from a widespread microbial virulence factor triggers pattern-induced immunity in Arabidopsis.PLoS pathogens · 2014Article
- Stem-cell-triggered immunity safeguards cytokinin enriched plant shoot apexes from pathogen infection.Frontiers in plant science · 2014Article
- Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility.The New phytologist · 2013Article
- Recognition of bacterial plant pathogens: local, systemic and transgenerational immunity.The New phytologist · 2013Review
- MAMP (microbe-associated molecular pattern) triggered immunity in plants.Frontiers in plant science · 2013Article
- The shoot apical meristem regulatory peptide CLV3 does not activate innate immunity.The Plant cell · 2012Article
- Commentaries and letters to the editor of the plant cell.The Plant cell · 2012Article
- Small signaling peptides in Arabidopsis development: how cells communicate over a short distance.The Plant cell · 2012Review
- Complexity in differential peptide-receptor signaling: response to Segonzac et Al. and Mueller et Al. commentaries.The Plant cell · 2012Article
- Chimeric FLS2 receptors reveal the basis for differential flagellin perception in Arabidopsis and tomato.The Plant cell · 2012Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The pattern recognition receptor FLAGELLIN SENSING2 (FLS2) renders plant cells responsive to subnanomolar concentrations of flg22, the active epitope of bacterial flagellin. We recently observed that a preparation of the peptide IDL1, a signal known to regulate abscission processes via the receptor kinases HAESA and HAESA-like2, apparently triggered Arabidopsis thaliana cells in an FLS2-dependent manner. However, closer investigation revealed that this activity was due to contamination by a flg22-type peptide, and newly synthesized IDL1 peptide was completely inactive in FLS2 signaling. This raised alert over contamination events occurring in the process of synthesis or handling of peptides. Two recent reports have suggested that FLS2 has further specificities for structurally unrelated peptides derived from CLV3 and from Ax21. We thus scrutinized these peptides for activity in Arabidopsis cells as well. While responding to <1 nM flg22, Arabidopsis cells proved blind even to 100 μM concentrations of CLV3p and axY(s)22. Our results confirm the exquisite sensitivity and selectivity of FLS2 for flg22. They also show that inadvertent contaminations with flg22-type peptides do occur and can be detected even in trace amounts by FLS2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.