ArticleThe Journal of biological chemistry2012
Characterization of the DNA-binding properties of the Mohawk homeobox transcription factor.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- IRX3 controls a SUMOylation-dependent differentiation switch in adipocyte precursor cells.Nature communications · 2025Article
- Experimental approaches to investigate biophysical interactions between homeodomain transcription factors and DNA.Biochimica et biophysica acta. Gene regulatory mechanisms · 2025Review
- Characterization of PRDM9 Multifunctionality in Yak Testes Through Protein Interaction Mapping.International journal of molecular sciences · 2025Article
- The Mohawk homeobox gene represents a marker and osteo-inhibitory factor in calvarial suture osteoprogenitor cells.Cell death & disease · 2024Article
- The Transcription Factor Mohawk Facilitates Skeletal Muscle Repair via Modulation of the Inflammatory Environment.International journal of molecular sciences · 2024Article
- Characterization of mRNA Signature in Milk Small Extracellular Vesicles from Cattle Infected with Bovine Leukemia Virus.Pathogens (Basel, Switzerland) · 2023Article
- External stimulation: A potential therapeutic strategy for tendon-bone healing.Frontiers in bioengineering and biotechnology · 2023Review
- Mohawk impedes angiofibrosis by preventing the differentiation of tendon stem/progenitor cells into myofibroblasts.Scientific reports · 2022Article
- Hemocyte Clusters Defined by scRNA-Seq inFrontiers in immunology · 2022Article
- Gene Co-expression Analysis Indicates Potential Pathways and Regulators of Beef Tenderness in Nellore Cattle.Frontiers in genetics · 2018Article
- Molecular studies revealOncology letters · 2017Article
- Gene targeting of the transcription factor Mohawk in rats causes heterotopic ossification of Achilles tendon via failed tenogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing.Nature communications · 2016Article
- Tendon development and musculoskeletal assembly: emerging roles for the extracellular matrix.Development (Cambridge, England) · 2015Review
- The Mkx homeoprotein promotes tenogenesis in stem cells and improves tendon repair.Annals of translational medicine · 2015Article
- Article
- Transcription factor Mohawk controls tenogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2015Article
- Deep sequencing of large library selections allows computational discovery of diverse sets of zinc fingers that bind common targets.Nucleic acids research · 2014Article
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The homeobox transcription factor Mohawk (Mkx) is a potent transcriptional repressor expressed in the embryonic precursors of skeletal muscle, cartilage, and bone. MKX has recently been shown to be a critical regulator of musculoskeletal tissue differentiation and gene expression; however, the genetic pathways through which MKX functions and its DNA-binding properties are currently unknown. Using a modified bacterial one-hybrid site selection assay, we determined the core DNA-recognition motif of the mouse monomeric Mkx homeodomain to be A-C-A. Using cell-based assays, we have identified a minimal Mkx-responsive element (MRE) located within the Mkx promoter, which is composed of a highly conserved inverted repeat of the core Mkx recognition motif. Using the minimal MRE sequence, we have further identified conserved MREs within the locus of Sox6, a transcription factor that represses slow fiber gene expression during skeletal muscle differentiation. Real-time PCR and immunostaining of in vitro differentiated muscle satellite cells isolated from Mkx-null mice revealed an increase in the expression of Sox6 and down-regulation of slow fiber structural genes. Together, these data identify the unique DNA-recognition properties of MKX and reveal a novel role for Mkx in promoting slow fiber type specification during skeletal muscle differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.