ArticleJournal of bacteriology2012
Two systems for conditional gene expression in Myxococcus xanthus inducible by isopropyl-β-D-thiogalactopyranoside or vanillate.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed.
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- Genetic and environmental determinants of streaming and aggregation in Myxococcus xanthus.Scientific reports · 2025Article
- A novel mechanism for bacterial sporulation based on programmed peptidoglycan degradation.bioRxiv : the preprint server for biology · 2025Article
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- A lytic transglycosylase connects bacterial focal adhesion complexes to the peptidoglycan cell wall.eLife · 2024Article
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- Coordinated peptidoglycan synthases and hydrolases stabilize the bacterial cell wall.Nature communications · 2023Article
- Molecular basis and design principles of switchable front-rear polarity and directional migration in Myxococcus xanthus.Nature communications · 2023Article
- Ribonuclease D Processes a Small RNA Regulator of Multicellular Development in Myxobacteria.Genes · 2023Article
- Coenzyme BEnvironmental microbiology · 2022Article
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5 authors.
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Abstract
Conditional expression of a gene is a powerful tool to study its function and is typically achieved by placing the gene under the control of an inducible promoter. There is, however, a dearth of such inducible systems in Myxococcus xanthus, a well-studied prokaryotic model for multicellular development, cell differentiation, motility, and light response and a promising source of secondary metabolites. The few available systems have limitations, and exogenously based ones are unavailable. Here, we describe two new, versatile inducible systems for conditional expression of genes in M. xanthus. One employs isopropyl-β-d-thiogalactopyranoside (IPTG) as an inducer and is inspired by those successfully applied in some other bacteria. The other requires vanillate as an inducer and is based on the system developed originally for Caulobacter crescentus and recently adapted for mammalian cells. Both systems are robust, with essentially no expression in the absence of an inducer. Depending on the inducer and the amounts added, expression levels can be modulated such that either system can conditionally express genes, including ones that are essential and are required at high levels such as ftsZ. The two systems operate during vegetative growth as well as during M. xanthus development. Moreover, they can be used to simultaneously induce expression of distinct genes within the same cell. The conditional expression systems we describe substantially expand the genetic tool kit available for studying M. xanthus gene function and cellular biology.
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