ArticleApplied and environmental microbiology2012
Functional analysis of family GH36 α-galactosidases from Ruminococcus gnavus E1: insights into the metabolism of a plant oligosaccharide by a human gut symbiont.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- Trial
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- Exploring Causal Links Between Gut Microbiota and Geriatric Syndromes: A Two-Sample Mendelian Randomization Analysis.International journal of medical sciences · 2024Article
- Comparative genomics reveals extensive intra-species genetic divergence of the prevalent gut commensalMicrobial genomics · 2023Article
- Complete Genome Analysis ofMicroorganisms · 2023Article
- Ruminococcus gnavus: friend or foe for human health.FEMS microbiology reviews · 2023Review
- Association between Gut Microbiota and Emotional-Behavioral Symptoms in Children with Attention-Deficit/Hyperactivity Disorder.Journal of personalized medicine · 2022Article
- EDESIA: Plants, Food and Health: A cross-disciplinary PhD programme from crop to clinic.Nutrition bulletin · 2022Article
- Microbiota in mesenteric adipose tissue from Crohn's disease promote colitis in mice.Microbiome · 2021Article
- RadA, a MSCRAMM Adhesin of the Dominant SymbioteBiomolecules · 2021Article
- Stachyose triggers apoptotic like cell death in drought sensitive but not resilient plants.Scientific reports · 2021Article
- Sucrose 6Microbial genomics · 2019Article
- Characterization of Properties and Transglycosylation Abilities of Recombinant α-Galactosidase from Cold-Adapted Marine BacteriumMarine drugs · 2018Article
- The Interaction of the Gut Microbiota with the Mucus Barrier in Health and Disease in Human.Microorganisms · 2018Review
- Fecal microbiota in pouchitis and ulcerative colitis.World journal of gastroenterology · 2016Article
- Infant Early Gut Colonization by Lachnospiraceae: High Frequency of Ruminococcus gnavus.Frontiers in pediatrics · 2016Article
- Evaluating the Contribution of Gut Microbiota to the Variation of Porcine Fatness with the Cecum and Fecal Samples.Frontiers in microbiology · 2016Article
- Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation.Nature communications · 2015Article
- Production of a Highly Protease-Resistant Fungal α-Galactosidase in Transgenic Maize Seeds for Simplified Feed Processing.PloS one · 2015Article
- A single genus in the gut microbiome reflects host preference and specificity.The ISME journal · 2015Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Ruminococcus gnavus belongs to the 57 most common species present in 90% of individuals. Previously, we identified an α-galactosidase (Aga1) belonging to glycoside hydrolase (GH) family 36 from R. gnavus E1 (M. Aguilera, H. Rakotoarivonina, A. Brutus, T. Giardina, G. Simon, and M. Fons, Res. Microbiol. 163:14-21, 2012). Here, we identified a novel GH36-encoding gene from the same strain and termed it aga2. Although aga1 showed a very simple genetic organization, aga2 is part of an operon of unique structure, including genes putatively encoding a regulator, a GH13, two phosphotransferase system (PTS) sequences, and a GH32, probably involved in extracellular and intracellular sucrose assimilation. The 727-amino-acid (aa) deduced Aga2 protein shares approximately 45% identity with Aga1. Both Aga1 and Aga2 expressed in Escherichia coli showed strict specificity for α-linked galactose. Both enzymes were active on natural substrates such as melibiose, raffinose, and stachyose. Aga1 and Aga2 occurred as homotetramers in solution, as shown by analytical ultracentrifugation. Modeling of Aga1 and Aga2 identified key amino acids which may be involved in substrate specificity and stabilization of the α-linked galactoside substrates within the active site. Furthermore, Aga1 and Aga2 were both able to perform transglycosylation reactions with α-(1,6) regioselectivity, leading to the formation of product structures up to [Hex](12) and [Hex](8), respectively. We suggest that Aga1 and Aga2 play essential roles in the metabolism of dietary oligosaccharides and could be used for the design of galacto-oligosaccharide (GOS) prebiotics, known to selectively modulate the beneficial gut microbiota.
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