ArticleApplied and environmental microbiology2012
Survival and germination of Bacillus cereus spores without outgrowth or enterotoxin production during in vitro simulation of gastrointestinal transit.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
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- Ingestion of Bacillus cereus spores dampens the immune response to favor bacterial persistence.Nature communications · 2024Article
- In Vitro Human Gastrointestinal Tract Simulation Systems: A Panoramic Review.Probiotics and antimicrobial proteins · 2024Review
- Apple cider vinegar exhibits promising antibiofilm activity against multidrug-resistantOpen veterinary journal · 2024Article
- High Genetic Diversity and Virulence Potential inFoods (Basel, Switzerland) · 2023Article
- The Combination of In Vitro Assessment of Stress Tolerance Ability, Autoaggregation, and Vitamin B-Producing Ability for New Probiotic Strain Introduction.Microorganisms · 2022Article
- Mechanism of Antibacterial Enhancement and Drug Resistance Based on Smart Medical Imaging on Antibiotics.Computational and mathematical methods in medicine · 2022Article
- Enigmatic Pilus-Like Endospore Appendages ofInternational journal of molecular sciences · 2021Review
- Sporulation is dispensable for the vegetable-associated life cycle of the human pathogen Bacillus cereus.Microbial biotechnology · 2021Article
- Human microbiota modulation via QseC sensor kinase mediated in the Escherichia coli O104:H4 outbreak strain infection in microbiome model.BMC microbiology · 2021Article
- TheToxins · 2020Review
- Food Sensing: Detection ofBiosensors · 2020Review
- Predominant Mycotoxins, Pathogenesis, Control Measures, and Detection Methods in Fermented Pastes.Toxins · 2020Review
- Risk presented to minimally processed chilled foods by psychrotrophicTrends in food science & technology · 2019Review
- Endospores and other lysis-resistant bacteria comprise a widely shared core community within the human microbiota.The ISME journal · 2018Article
- Lineage-specific plasmid acquisition and the evolution of specialized pathogens in Bacillus thuringiensis and the Bacillus cereus group.Molecular ecology · 2018Article
- Superdormant Spores as a Hurdle for Gentle Germination-Inactivation Based Spore Control Strategies.Frontiers in microbiology · 2018Article
- In defense of Bacillus thuringiensis, the safest and most successful microbial insecticide available to humanity - a response to EFSA.FEMS microbiology ecology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the gastrointestinal survival and enterotoxin production of the food-borne pathogen Bacillus cereus, an in vitro simulation experiment was developed to mimic gastrointestinal passage in 5 phases: (i) the mouth, (ii) the stomach, with gradual pH decrease and fractional emptying, (iii) the duodenum, with high concentrations of bile and digestive enzymes, (iv) dialysis to ensure bile reabsorption, and (v) the ileum, with competing human intestinal bacteria. Four different B. cereus strains were cultivated and sporulated in mashed potato medium to obtain an inoculum of 7.0 log spores/ml. The spores showed survival and germination during the in vitro simulation of gastrointestinal passage, but vegetative outgrowth of the spores was suppressed by the intestinal bacteria during the final ileum phase. No bacterial proliferation or enterotoxin production was observed, despite the high inoculum levels. Little strain variability was observed: except for the psychrotrophic food isolate, the spores of all strains survived well throughout the gastrointestinal passage. The in vitro simulation experiments investigated the survival and enterotoxin production of B. cereus in the gastrointestinal lumen. The results obtained support the hypothesis that localized interaction of B. cereus with the host's epithelium is required for diarrheal food poisoning.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.