ArticleJournal of innate immunity2013
Extracellular DNA within a nontypeable Haemophilus influenzae-induced biofilm binds human beta defensin-3 and reduces its antimicrobial activity.
Article in Journal of innate immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 89 citations in OpenAlex.
- Natural Anti-Biofilm Agents: A Comprehensive Review and Future Perspectives.Current microbiology · 2025Review
- Extracellular defense of bacteria against antimicrobial peptides.Journal of bacteriology · 2025Review
- Exploring the Antibiotic Potential of a Serine Protease fromInfectious disease reports · 2025Article
- Extracellular DNA-protein interactions.Current opinion in structural biology · 2024Review
- Secreted nucleases reclaim extracellular DNA during biofilm development.NPJ biofilms and microbiomes · 2024Article
- Antimicrobial peptide-based strategies to overcome antimicrobial resistance.Archives of microbiology · 2024Review
- Quantitative Imaging of the Action of vCPP2319, an Antimicrobial Peptide from a Viral Scaffold, againstACS infectious diseases · 2023Article
- Combating Bacterial Biofilms: Current and Emerging Antibiofilm Strategies for Treating Persistent Infections.Antibiotics (Basel, Switzerland) · 2023Review
- Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies.Infection and drug resistance · 2023Review
- The Roles of NOD-like Receptors in Innate Immunity in Otitis Media.International journal of molecular sciences · 2022Review
- Article
- The Two Weapons against Bacterial Biofilms: Detection and Treatment.Antibiotics (Basel, Switzerland) · 2021Review
- Extracellular DNA (eDNA). A Major Ubiquitous Element of the Bacterial Biofilm Architecture.International journal of molecular sciences · 2021Review
- Molecular engineering of antimicrobial peptides: microbial targets, peptide motifs and translation opportunities.Biophysical reviews · 2021Review
- Article
- The Role of Non-TypeableFrontiers in cellular and infection microbiology · 2021Review
- DNA Blocks the Lethal Effect of Human Beta-Defensin 2 AgainstFrontiers in microbiology · 2021Article
- Targeting Biofilms Therapy: Current Research Strategies and Development Hurdles.Microorganisms · 2020Review
- Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens.Frontiers in microbiology · 2020Review
- Subversion of host immune responses by otopathogens during otitis media.Journal of leukocyte biology · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Biofilms formed by nontypeable Haemophilus influenzae (NTHI) are associated with multiple chronic infections of the airway, including otitis media. Extracellular DNA (eDNA) is part of the biofilm matrix and serves as a structural component. Human β-defensin-3 (hBD-3) is a cationic antimicrobial host defense protein (AMP) critical to the protection of the middle ear. We hypothesized that anionic eDNA could interact with and bind hBD-3 and thus shield NTHI in biofilms from its antimicrobial activity. We demonstrated that recombinant hBD-3 [(r)hBD-3] bound eDNA in vitro and that eDNA in biofilms produced by NTHI in the chinchilla middle ear co-localized with the orthologue of this AMP. Incubation of physiological concentrations of (r)hBD-3 with NTHI genomic DNA abrogated the ability of this innate immune effector to prevent NTHI from forming robust biofilms in vitro. Establishment of NTHI biofilms in the presence of both DNase I and (r)hBD-3 resulted in a marked reduction in the overall height and thickness of the biofilms and rescued the antimicrobial activity of the AMP. Our results demonstrated that eDNA in NTHI biofilms sequestered hBD-3 and thus diminished the biological activity of an important effector of innate immunity. Our observations have important implications for chronicity of NTHI-induced diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.