ArticleJournal of controlled release : official journal of the Controlled Release Society2012
Microneedle-mediated transcutaneous immunization with plasmid DNA coated on cationic PLGA nanoparticles.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Microneedles for Vaccination: Mechanistic Foundations, Materials Innovation, Clinical Translation, and Global Health Implementation.Pharmaceutics · 2026Review
- Smart micro/nanoneedles for gene delivery.Iranian journal of basic medical sciences · 2026Review
- Bimodal Dissolving Microneedles with Nanoparticle Coating and Encapsulation for Extended Dual-Drug Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Beyond the Needle: Innovative Microneedle-Based Transdermal Vaccination.Medicines (Basel, Switzerland) · 2025Review
- Recent Advancements in Microneedle Technology for Multifaceted Biomedical Applications.Pharmaceutics · 2022Review
- Microneedle systems for delivering nucleic acid drugs.Journal of pharmaceutical investigation · 2022Review
- Skin Penetration Enhancement Strategies Used in the Development of Melanoma Topical Treatments.The AAPS journal · 2021Review
- Microneedle Arrays Combined with Nanomedicine Approaches for Transdermal Delivery of Therapeutics.Journal of clinical medicine · 2021Review
- Progress in microneedle array patch (MAP) for vaccine delivery.Human vaccines & immunotherapeutics · 2021Article
- Design and immunological evaluation of anti-CD205-tailored PLGA-based nanoparticulate cancer vaccine.International journal of nanomedicine · 2018Article
- Article
- Self-Assembly DNA Polyplex Vaccine inside Dissolving Microneedles for High-Potency Intradermal Vaccination.Theranostics · 2017Article
- A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin.International journal of nanomedicine · 2017Article
- A method to improve the efficacy of topical eflornithine hydrochloride cream.Drug delivery · 2016Article
- Induction of protective neutralizing antibody responses against botulinum neurotoxin serotype C using plasmid carried by PLGA nanoparticles.Human vaccines & immunotherapeutics · 2016Article
- Microneedles: A New Frontier in Nanomedicine Delivery.Pharmaceutical research · 2016Review
- Microneedles As a Delivery System for Gene Therapy.Frontiers in pharmacology · 2016Review
- Messenger RNA (mRNA) nanoparticle tumour vaccination.Nanoscale · 2014Review
- Microneedle delivery of an M2e-TLR5 ligand fusion protein to skin confers broadly cross-protective influenza immunity.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Nanomedicine-based neuroprotective strategies in patient specific-iPSC and personalized medicine.International journal of molecular sciences · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Previously, it was shown that microneedle-mediated transcutaneous immunization with plasmid DNA can potentially induce a stronger immune response than intramuscular injection of the same plasmid DNA. In the present study, we showed that the immune responses induced by transcutaneous immunization by applying plasmid DNA onto a skin area pretreated with solid microneedles were significantly enhanced by coating the plasmid DNA on the surface of cationic nanoparticles. In addition, the net surface charge of the DNA-coated nanoparticles significantly affected their in vitro skin permeation and their ability to induce immune responses in vivo. Transcutaneous immunization with plasmid DNA-coated net positively charged nanoparticles elicited a stronger immune response than with plasmid DNA-coated net negatively charged nanoparticles or by intramuscular immunization with plasmid DNA alone. Transcutaneous immunization with plasmid DNA-coated net positively charged nanoparticles induced comparable immune responses as intramuscular injection of them, but transcutaneous immunization was able to induce specific mucosal immunity and a more balanced T helper type 1 and type 2 response. The ability of the net positively charged DNA-coated nanoparticles to induce a strong immune response through microneedle-mediated transcutaneous immunization may be attributed to their ability to increase the expression of the antigen gene encoded by the plasmid and to more effectively stimulate the maturation of antigen-presenting cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.