ArticleFrontiers in cellular and infection microbiology2012
Polynucleotide phosphorylase has an impact on cell biology of Campylobacter jejuni.
Article in Frontiers in cellular and infection microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 33 citations in OpenAlex.
- Identification of Ribonuclease Inhibitors for the Control of Pathogenic Bacteria.International journal of molecular sciences · 2024Article
- Developing New Tools to Fight Human Pathogens: A Journey through the Advances in RNA Technologies.Microorganisms · 2022Review
- PNPase is involved in the coordination of mRNA degradation and expression in stationary phase cells of Escherichia coli.BMC genomics · 2018Article
- Regulatory RNAs in Virulence and Host-Microbe Interactions.Microbiology spectrum · 2018Review
- The Role of Ribonucleases and sRNAs in the Virulence of Foodborne Pathogens.Frontiers in microbiology · 2017Review
- Profiling ofFrontiers in microbiology · 2017Article
- The small RNA SraG participates in PNPase homeostasis.RNA (New York, N.Y.) · 2016Article
- Insights into Campylobacter jejuni colonization and enteritis using a novel infant rabbit model.Scientific reports · 2016Article
- Neisseria meningitidis Polynucleotide Phosphorylase Affects Aggregation, Adhesion, and Virulence.Infection and immunity · 2016Article
- The impact of environmental conditions on Campylobacter jejuni survival in broiler faeces and litter.Infection ecology & epidemiology · 2016Article
- Adhesion, Biofilm Formation, and Genomic Features of Campylobacter jejuni Bf, an Atypical Strain Able to Grow under Aerobic Conditions.Frontiers in microbiology · 2016Article
- Induction of osmoadaptive mechanisms and modulation of cellular physiology help Bacillus licheniformis strain SSA 61 adapt to salt stress.Current microbiology · 2015Article
- Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli.BMC genomics · 2015Article
- Defects in polynucleotide phosphorylase impairs virulence in Escherichia coli O157:H7.Frontiers in microbiology · 2015Article
- The RNase R from Campylobacter jejuni has unique features and is involved in the first steps of infection.The Journal of biological chemistry · 2014Article
- Characterization of the biochemical properties of Campylobacter jejuni RNase III.Bioscience reports · 2013Article
- The exoribonuclease Polynucleotide Phosphorylase influences the virulence and stress responses of yersiniae and many other pathogens.Frontiers in cellular and infection microbiology · 2013Review
- Exoribonucleases as modulators of virulence in pathogenic bacteria.Frontiers in cellular and infection microbiology · 2012Article
- Research advances in the study of Campylobacter, Helicobacter, and related organisms.Frontiers in cellular and infection microbiology · 2012Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polynucleotide phosphorylase (PNPase), encoded by the pnp gene, is known to degrade mRNA, mediating post-transcriptional regulation and may affect cellular functions. The role of PNPase is pleiotropic. As orthologs of the two major ribonucleases (RNase E and RNase II) of Escherichia coli are missing in the Campylobacter jejuni genome, in the current study the focus has been on the C. jejuni ortholog of PNPase. The effect of PNPase mutation on C. jejuni phenotypes and proteome was investigated. The inactivation of the pnp gene reduced significantly the ability of C. jejuni to adhere and to invade Ht-29 cells. Moreover, the pnp mutant strain exhibited a decrease in C. jejuni swimming ability and chick colonization. To explain effects of PNPase on C. jejuni 81-176 phenotype, the proteome of the pnp mutant and parental strains were compared. Overall, little variation in protein production was observed. Despite the predicted role of PNPase in mRNA regulation, the pnp mutation did not induce profound proteomic changes suggesting that other ribonucleases in C. jejuni might ensure this biological function in the absence of PNPase. Nevertheless, synthesis of proteins which are involved in virulence (LuxS, PEB3), motility (N-acetylneuraminic acid synthetase), stress-response (KatA, DnaK, Hsp90), and translation system (EF-Tu, EF-G) were modified in the pnp mutant strain suggesting a more specific role of PNPase in C. jejuni. In conclusion, PNPase deficiency induces limited but important consequences on C. jejuni biology that could explain swimming limitation, chick colonization delay, and the decrease of cell adhesion/invasion ability.
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