ArticleThe journal of physical chemistry letters2012
Fast Detection Allows Analysis of the Electronic Structure of Metalloprotein by X-ray Emission Spectroscopy at Room Temperature.
Article in The journal of physical chemistry letters, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 25 citations in OpenAlex.
- Probing soft X-ray induced photoreduction of a model Mn-complex at cryogenic conditions.Journal of synchrotron radiation · 2025Article
- Probing Physical Oxidation State by Resonant X-ray Emission Spectroscopy: Applications to Iron Model Complexes and Nitrogenase.Angewandte Chemie (International ed. in English) · 2021Article
- Quantification of Ni-N-O Bond Angles and NO Activation by X-ray Emission Spectroscopy.Inorganic chemistry · 2021Article
- X-ray-induced sample damage at the Mn L-edge: a case study for soft X-ray spectroscopy of transition metal complexes in solution.Physical chemistry chemical physics : PCCP · 2018Article
- AXSIS: Exploring the frontiers in attosecond X-ray science, imaging and spectroscopy.Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment · 2016Article
- Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.Nature methods · 2014Article
- Experimentally quantifying small-molecule bond activation using valence-to-core X-ray emission spectroscopy.Journal of the American Chemical Society · 2013Article
- Kinetic modeling of the X-ray-induced damage to a metalloprotein.The journal of physical chemistry. B · 2013Article
- DIY XES - development of an inexpensive, versatile, and easy to fabricate XES analyzer and sample delivery system.X-ray spectrometry : XRSArticle
- Rapid evolution of the Photosystem II electronic structure during water splitting.Physical review. XArticle
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
The paradigm of "detection-before-destruction" was tested for a metalloprotein complex exposed at room temperature to the high x-ray flux typical of third generation synchrotron sources. Following the progression of the x-ray induced damage by Mn Kβ x-ray emission spectroscopy, we demonstrated the feasibility of collecting room temperature data on the electronic structure of native Photosystem II, a trans-membrane metalloprotein complex containing a Mn(4)Ca cluster. The determined non-damaging observation timeframe (about 100 milliseconds using continuous monochromatic beam, deposited dose 1*10(7) photons/µm(2) or 1.3*10(4) Gy, and 66 microseconds in pulsed mode using pink beam, deposited dose 4*10(7) photons/µm(2) or 4.2*10(4) Gy) is sufficient for the analysis of this protein's electron dynamics and catalytic mechanism at room temperature. Reported time frames are expected to be representative for other metalloproteins. The described instrumentation, based on the short working distance dispersive spectrometer, and experimental methodology is broadly applicable to time-resolved x-ray emission analysis at synchrotron and x-ray free-electron laser light sources.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.