ArticleBiophysical journal1990
Binding of a monoclonal antibody and its Fab fragment to supported phospholipid monolayers measured by total internal reflection fluorescence microscopy.
Article in Biophysical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 80 citations in OpenAlex.
- Review
- Measuring Binding Kinetics of Antibody-Conjugated Gold Nanoparticles with Intact Cells.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Rifampicin-independent interactions between the pregnane X receptor ligand binding domain and peptide fragments of coactivator and corepressor proteins.Biochemistry · 2012Article
- Using covalent dimers of human carbonic anhydrase II to model bivalency in immunoglobulins.Journal of the American Chemical Society · 2011Article
- Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerations.The journal of physical chemistry. B · 2011Article
- Multivalent ligand-receptor binding on supported lipid bilayers.Journal of structural biology · 2009Review
- Impact of hapten presentation on antibody binding at lipid membrane interfaces.Biophysical journal · 2008Article
- Engineering and rapid selection of a low-affinity glucose/galactose-binding protein for a glucose biosensor.Protein science : a publication of the Protein Society · 2007Article
- GM1 clustering inhibits cholera toxin binding in supported phospholipid membranes.Journal of the American Chemical Society · 2007Article
- Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusion.Biophysical journal · 2001Article
- Lipid rafts reconstituted in model membranes.Biophysical journal · 2001Article
- Molecular dynamics simulations of supported phospholipid/alkanethiol bilayers on a gold(111) surface.Biophysical journal · 1999Article
- Imaging fluorescence correlation spectroscopy: nonuniform IgE distributions on planar membranes.Biophysical journal · 1996Article
- Surface engineering: optimization of antigen presentation in self-assembled monolayers.Biophysical journal · 1996Article
- Theory for measuring bivalent surface binding kinetics using total internal reflection with fluorescence photobleaching recovery.Biophysical journal · 1994Article
- Influence of surface chemistry on the structural organization of monomolecular protein layers adsorbed to functionalized aqueous interfaces.Biophysical journal · 1993Article
- Total internal reflection fluorescence microscopy: application to substrate-supported planar membranes.European biophysics journal : EBJ · 1993Review
- Binding kinetics of an anti-dinitrophenyl monoclonal Fab on supported phospholipid monolayers measured by total internal reflection with fluorescence photobleaching recovery.Biophysical journal · 1992Article
- Evanescent interference patterns for fluorescence microscopy.Biophysical journal · 1992Article
- Measurement of mass transport and reaction parameters in bulk solution using photobleaching. Reaction limited binding regime.Biophysical journal · 1991Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The association of an anti-dinitrophenyl monoclonal antibody and its Fab fragment with supported phospholipid monolayers composed of a mixture of dipalmitoylphosphatidylcholine and dinitrophenyl-conjugated dipalmitoylphosphatidylethanolamine has been characterized with total internal reflection fluorescence microscopy. The surface densities of bound antibodies were measured as a function of the antibody and Fab solution concentrations, and as a function of the solution concentration of dinitrophenylglycine. The apparent association constant of Fab fragments with surface-associated haptens was approximately 10-fold lower than the association constant for haptens in solution, and the apparent surface association constant for intact antibodies was only approximately 10-fold higher than the constant for Fab fragments. Data analysis with simple theoretical models indicated that, at most antibody surface densities, 50-90% of membrane-associated intact antibodies were attached to the surface by two antigen binding sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.