Evidence map›Paper›PMID 22919272›Full record

ArticleJournal of biomedicine & biotechnology2012

Distribution of genes and repetitive elements in the Diabrotica virgifera virgifera genome estimated using BAC sequencing.

Brad S Coates, Analiza P Alves, Haichuan Wang, Kimberly K O Walden, B Wade French, Nicholas J Miller, Craig A Abel, Hugh M Robertson, Thomas W Sappington, Blair D Siegfried

Open access · hybridAbstract read
In one paragraph

Article in Journal of biomedicine & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 19 citations in OpenAlex.

  1. Tracking Adaptive Pathways of Invasive Insects: Novel Insight from Genomics.International journal of molecular sciences · 2023
    Review
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  5. Draft Genome of a Blister BeetleFrontiers in genetics · 2019
    Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Brad S CoatesCorn Insects and Crop Genetics Research Unit, ARS, USDA, Ames, IA 50011, USA. brad.coates@ars.usda.gov
Analiza P Alves
Haichuan Wang
Kimberly K O Walden
B Wade French
Nicholas J Miller
Craig A Abel
Hugh M Robertson
Thomas W Sappington
Blair D Siegfried
University of Nebraska–Lincoln · USAgricultural Research Service · USUniversity of Illinois Urbana-Champaign · USNorth Central College · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feeding damage caused by the western corn rootworm, Diabrotica virgifera virgifera, is destructive to corn plants in North America and Europe where control remains challenging due to evolution of resistance to chemical and transgenic toxins. A BAC library, DvvBAC1, containing 109,486 clones with 104 ± 34.5 kb inserts was created, which has an ~4.56X genome coverage based upon a 2.58 Gb (2.80 pg) flow cytometry-estimated haploid genome size. Paired end sequencing of 1037 BAC inserts produced 1.17 Mb of data (~0.05% genome coverage) and indicated ~9.4 and 16.0% of reads encode, respectively, endogenous genes and transposable elements (TEs). Sequencing genes within BAC full inserts demonstrated that TE densities are high within intergenic and intron regions and contribute to the increased gene size. Comparison of homologous genome regions cloned within different BAC clones indicated that TE movement may cause haplotype variation within the inbred strain. The data presented here indicate that the D. virgifera virgifera genome is large in size and contains a high proportion of repetitive sequence. These BAC sequencing methods that are applicable for characterization of genomes prior to sequencing may likely be valuable resources for genome annotation as well as scaffolding.

Indexed as

AnimalsChromosomes, Artificial, BacterialColeopteraDNA Transposable ElementsElectrophoresis, Agar GelFlow CytometryGene LibraryGenes, InsectGenome SizeGenomicsHaplotypesMolecular Sequence AnnotationRepetitive Sequences, Nucleic AcidSequence Analysis, DNADNA Transposable Elements

Identifiers

PMID22919272
PMCPMC3420361
OpenAlexW2013473989

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.