ArticleGenome research2012
Analysis of gene expression from the Wolbachia genome of a filarial nematode supports both metabolic and defensive roles within the symbiosis.
Article in Genome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers, 1 of them a synthesis that pooled it.
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Who cites it
126 citing papers in PubMed, 1 synthesis or guideline pooled it, 194 citations in OpenAlex.
- A Meta-Analysis ofG3 (Bethesda, Md.) · 2020Pooled it
- Calibrating and documenting host-switching and evolution of incompatibility loci for two closely related Wolbachia clades.Genetics · 2026Article
- Calibrating and documenting host-switching and evolution of incompatibility loci for two closely relatedbioRxiv : the preprint server for biology · 2026Article
- Onchocerciasis overview: pathogenesis, clinical spectrum, and therapeutic challenges of a neglected tropical disease.Frontiers in parasitology · 2026Review
- Diversity of Bacteria.Progress in molecular and subcellular biology · 2026Review
- Microbiological and molecular profiling of human hydatid cysts reveals high frequency of Klebsiella species.BMC infectious diseases · 2025Article
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- Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiontMicrobial genomics · 2025Article
- Nucleotide composition shapes gene expression inmSystems · 2025Article
- Fexinidazole and Corallopyronin A target Wolbachia-infected sheath cells present in filarial nematodes.PLoS pathogens · 2025Article
- Master of Puppets: How Microbiota Drive the Nematoda Ecology and Evolution?Ecology and evolution · 2025Review
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- In vitro molting of Dirofilaria immitis third-stage larvae derived from microfilariae collected from doxycycline-treated dogs.Parasitology research · 2025Article
- Review
- Real-time PCR and immunohistochemistry detection of Wolbachia in adult Dirofilaria immitis from dogs treated with doxycycline and ivermectin.Parasites & vectors · 2025Article
- Fexinidazole and Corallopyronin A targetbioRxiv : the preprint server for biology · 2025Article
- Improved RNA preparation for RNA-seq of the intracellular bacteriumFrontiers in microbiology · 2025Article
- UnveilingFrontiers in cellular and infection microbiology · 2025Article
- Unveiling Diversity and Function: Venom-Associated Microbes in Two Spiders, Heteropoda venatoria and Chilobrachys guangxiensis.Microbial ecology · 2024Article
- Metatranscriptomics provide insights into the role of the symbiont Midichloria mitochondrii in Ixodes ticks.FEMS microbiology ecology · 2024Article
66 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The α-proteobacterium Wolbachia is probably the most prevalent, vertically transmitted symbiont on Earth. In contrast with its wide distribution in arthropods, Wolbachia is restricted to one family of animal-parasitic nematodes, the Onchocercidae. This includes filarial pathogens such as Onchocerca volvulus, the cause of human onchocerciasis, or river blindness. The symbiosis between filariae and Wolbachia is obligate, although the basis of this dependency is not fully understood. Previous studies suggested that Wolbachia may provision metabolites (e.g., haem, riboflavin, and nucleotides) and/or contribute to immune defense. Importantly, Wolbachia is restricted to somatic tissues in adult male worms, whereas females also harbor bacteria in the germline. We sought to characterize the nature of the symbiosis between Wolbachia and O. ochengi, a bovine parasite representing the closest relative of O. volvulus. First, we sequenced the complete genome of Wolbachia strain wOo, which revealed an inability to synthesize riboflavin de novo. Using RNA-seq, we also generated endobacterial transcriptomes from male soma and female germline. In the soma, transcripts for membrane transport and respiration were up-regulated, while the gonad exhibited enrichment for DNA replication and translation. The most abundant Wolbachia proteins, as determined by geLC-MS, included ligands for mammalian Toll-like receptors. Enzymes involved in nucleotide synthesis were dominant among metabolism-related proteins, whereas the haem biosynthetic pathway was poorly represented. We conclude that Wolbachia may have a mitochondrion-like function in the soma, generating ATP for its host. Moreover, the abundance of immunogenic proteins in wOo suggests a role in diverting the immune system toward an ineffective antibacterial response.
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