ArticleRNA (New York, N.Y.)2012
Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway.
Article in RNA (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Mettl1-dependent mNature communications · 2024Article
- Perturbation of METTL1-mediated tRNA NNature communications · 2024Article
- Maf1 controls retinal neuron number by both RNA Pol III- and Pol II-dependent mechanisms.iScience · 2023Article
- The life and times of a tRNA.RNA (New York, N.Y.) · 2023Review
- Hypomodified tRNA in evolutionarily distant yeasts can trigger rapid tRNA decay to activate the general amino acid control response, but with different consequences.PLoS genetics · 2020Article
- Mutations in the anticodon stem of tRNA cause accumulation and Met22-dependent decay of pre-tRNA in yeast.RNA (New York, N.Y.) · 2020Article
- Glycolytic flux inThe Biochemical journal · 2019Article
- Article
- The RNA degradosome promotes tRNA quality control through clearance of hypomodified tRNA.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- 7-Methylguanosine Modifications in Transfer RNA (tRNA).International journal of molecular sciences · 2018Review
- Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions.Science signaling · 2018Article
- Noncoding RNA Surveillance: The Ends Justify the Means.Chemical reviews · 2018Review
- tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Review
- Small RNA fragments derived from multiple RNA classes - the missing element of multi-omics characteristics of the hepatitis C virus cell culture model.BMC genomics · 2017Article
- Transcription by RNA polymerase III: insights into mechanism and regulation.Biochemical Society transactions · 2016Review
- Global translational impacts of the loss of the tRNA modification tMicrobial cell (Graz, Austria) · 2016Article
- Surveillance and cleavage of eukaryotic tRNAs.International journal of molecular sciences · 2015Review
- Transfer RNA post-transcriptional processing, turnover, and subcellular dynamics in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- Controlling translation via modulation of tRNA levels.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
tRNA precursors, which are transcribed by RNA polymerase III, undergo end-maturation, splicing, and base modifications. Hypomodified tRNAs, such as tRNA(Val(AAC)), lacking 7-methylguanosine and 5-methylcytidine modifications, are subject to degradation by a rapid tRNA decay pathway. Here we searched for genes which, when overexpressed, restored stability of tRNA(Val(AAC)) molecules in a modification-deficient trm4Δtrm8Δ mutant. We identified TEF1 and VAS1, encoding elongation factor eEF1A and valyl-tRNA synthetase respectively, which likely protect hypomodified tRNA(Val(AAC)) by direct interactions. We also identified MAF1 whose product is a general negative regulator of RNA polymerase III. Expression of a Maf1-7A mutant that constitutively repressed RNA polymerase III transcription resulted in increased stability of hypomodified tRNA(Val(AAC)). Strikingly, inhibition of tRNA transcription in a Maf1-independent manner, either by point mutation in RNA polymerase III subunit Rpc128 or decreased expression of Rpc17 subunit, also suppressed the turnover of the hypomodified tRNA(Val(AAC)). These results support a model where inhibition of tRNA transcription leads to stabilization of hypomodified tRNA(Val(AAC)) due to more efficient protection by tRNA-interacting proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.