ArticleAngiogenesis2012
An in vivo neovascularization assay for screening regulators of angiogenesis and assessing their effects on pre-existing vessels.
Article in Angiogenesis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- The Effect of TISSEELMedicina (Kaunas, Lithuania) · 2026Article
- Vascularization potential of a dermal skin substitute material (Biodegradable Temporizing Matrix) by proangiogenic growth factors and ASC - an in ovo study.Journal of materials science. Materials in medicine · 2025Article
- Adjuvant-free immunization with infective filarial larvae as lymphatic homing antigen carriers.Scientific reports · 2020Article
- Consensus guidelines for the use and interpretation of angiogenesis assays.Angiogenesis · 2018Review
- Physiological Perspective on Therapies of Lymphatic Vessels.Advances in wound care · 2018Review
- Anti-angiogenic drug loaded liposomes: Nanotherapy for early atherosclerotic lesions in mice.PloS one · 2018Article
- Direct imaging of capillaries reveals the mechanism of arteriovenous interlacing in the chick chorioallantoic membrane.Communications biology · 2018Article
- Strategies Developed to Induce, Direct, and Potentiate Bone Healing.Frontiers in physiology · 2017Review
- Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model.Stem cells international · 2015Article
- Long-term intravital immunofluorescence imaging of tissue matrix components with epifluorescence and two-photon microscopy.Journal of visualized experiments : JoVE · 2014Article
- Optimization and regeneration kinetics of lymphatic-specific photodynamic therapy in the mouse dermis.Angiogenesis · 2014Article
- Deciphering combinations of PI3K/AKT/mTOR pathway drugs augmenting anti-angiogenic efficacy in vivo.PloS one · 2014Article
- Article
- Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don.BMC complementary and alternative medicine · 2013Article
- Intravital immunofluorescence for visualizing the microcirculatory and immune microenvironments in the mouse ear dermis.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Therapeutic regulation of tissue vascularization has appeared as an attractive approach to treat a number of human diseases. In vivo neovascularization assays that reflect physiological and pathological formation of neovessels are important in this effort. In this report we present an assay where the effects of activators and inhibitors of angiogenesis can be quantitatively and qualitatively measured. A provisional matrix composed of collagen I and fibrin was formed in a plastic cylinder and implanted onto the chick chorioallantoic membrane. A nylon mesh separated the implanted matrix from the underlying tissue to distinguish new from pre-existing vessels. Vascularization of the matrix in response to fibroblast growth factor-2 or platelet-derived growth factor-BB was scored in a double-blinded manner, or vessel density was measured using a semi-automated image analysis procedure. Thalidomide, fumagillin, U0126 and TGFβ inhibited neovessel growth while hydrocortisone exerted a negative and wortmannin a toxic effect on the pre-existing vasculature. This quantitative, inexpensive and rapid in vivo angiogenesis assay might be a valuable tool in screening and characterizing factors that influence wound or tumor induced vascularization and in assessing their effects on the normal vasculature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.