ArticlePloS one2012
ARF6-dependent regulation of P2Y receptor traffic and function in human platelets.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- ARF6 inhibition stabilizes the vasculature and enhances survival during endotoxic shock.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- Histopathological Assessment of Myocardial Ischemia-Reperfusion Injury Using Transformer-Based Artificial Intelligence: Model Comparison Study.JMIR medical informatics · 2026Article
- Roles of G proteins and their GTPase-activating proteins in platelets.Bioscience reports · 2024Review
- New insights of platelet endocytosis and its implication for platelet function.Frontiers in cardiovascular medicine · 2023Review
- Artificial-Intelligence-Assisted Discovery of Genetic Factors for Precision Medicine of Antiplatelet Therapy in Diabetic Peripheral Artery Disease.Biomedicines · 2022Article
- The small GTPase ARF6 regulates protein trafficking to control cellular function during development and in disease.Small GTPases · 2019Article
- Small GTPases in platelet membrane trafficking.Platelets · 2019Review
- The ins and outs of endocytic trafficking in platelet functions.Current opinion in hematology · 2017Review
- Platelet secretion in inflammatory and infectious diseases.Platelets · 2017Review
- Platelets in Pulmonary Immune Responses and Inflammatory Lung Diseases.Physiological reviews · 2016Review
- Arf6 arbitrates fibrinogen endocytosis.Blood · 2016Article
- Article
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
- Exchange factor EFA6R requires C-terminal targeting to the plasma membrane to promote cytoskeletal rearrangement through the activation of ADP-ribosylation factor 6 (ARF6).The Journal of biological chemistry · 2014Article
- Cytohesin-2 phosphorylation by protein kinase C relieves the constitutive suppression of platelet dense granule secretion by ADP-ribosylation factor 6.Journal of thrombosis and haemostasis : JTH · 2014Article
- Impact of cigarette smoking on P2Y12 receptor binding activity before and after clopidogrel therapy in patients with coronary artery disease.Journal of cardiovascular translational research · 2014Article
- Rab family proteins regulate the endosomal trafficking and function of RGS4.The Journal of biological chemistry · 2013Article
- Platelets as cellular effectors of inflammation in vascular diseases.Circulation research · 2013Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Adenosine diphosphate (ADP) is a critical regulator of platelet activation, mediating its actions through two G protein-coupled receptors, the P2Y(1) and P2Y(12) purinoceptors. Recently, we demonstrated that P2Y(1) and P2Y(12) purinoceptor activities are rapidly and reversibly modulated in human platelets, revealing that the underlying mechanism requires receptor internalization and subsequent trafficking as an essential part of this process. In this study we investigated the role of the small GTP-binding protein ADP ribosylation factor 6 (ARF6) in the internalization and function of P2Y(1) and P2Y(12) purinoceptors in human platelets. ARF6 has been implicated in the internalization of a number of GPCRs, although its precise molecular mechanism in this process remains unclear. In this study we show that activation of either P2Y(1) or P2Y(12) purinoceptors can stimulate ARF6 activity. Further blockade of ARF6 function either in cell lines or human platelets blocks P2Y purinoceptor internalization. This blockade of receptor internalization attenuates receptor resensitization. Furthermore, we demonstrate that Nm23-H1, a nucleoside diphosphate (NDP) kinase regulated by ARF6 which facilitates dynamin-dependent fission of coated vesicles during endocytosis, is also required for P2Y purinoceptor internalization. These data describe a novel function of ARF6 in the internalization of P2Y purinoceptors and demonstrate the integral importance of this small GTPase upon platelet ADP receptor function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.