ArticleBlood2012
Microfluidic kit-on-a-lid: a versatile platform for neutrophil chemotaxis assays.
Article in Blood, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02411318 (Influence of Lifestyle Factors on Neutrophil Migration Pilot Study), which is not on this map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Influence of Lifestyle Factors on Neutrophil Migration Pilot Study
Who cites it
39 citing papers in PubMed, 80 citations in OpenAlex.
- Exclusive liquid repellency isolation (ELRi) microliter scale isolation technique unmasks T-cell migration defects to CCL21 in pediatric asthma.bioRxiv : the preprint server for biology · 2026Article
- Microliter Whole Blood Neutrophil Assay Preserving Physiological Lifespan and Functional Heterogeneity.Small methods · 2024Article
- Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.Lab on a chip · 2024Article
- [Inertial label-free sorting and chemotaxis of polymorphonuclear neutrophil in sepsis patients based on microfluidic technology].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2023Article
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Simplified Cell Magnetic Isolation Assisted SCMicromachines · 2022Article
- Method for simultaneous tracking of thousands of unlabeled cells within a transparent 3D matrix.PloS one · 2022Article
- Bacterial Infection-Mimicking Three-Dimensional Phagocytosis and Chemotaxis in Electrospun Poly(ε-caprolactone) Nanofibrous Membrane.Membranes · 2021Article
- Microphysiological Systems for Studying Cellular Crosstalk During the Neutrophil Response to Infection.Frontiers in immunology · 2021Review
- Insights Into Leukocyte Trafficking in Inflammatory Arthritis - Imaging the Joint.Frontiers in cell and developmental biology · 2021Review
- Microfluidic devices for neutrophil chemotaxis studies.Journal of translational medicine · 2020Review
- Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa.Analytical chemistry · 2020Article
- Towards the development of human immune-system-on-a-chip platforms.Drug discovery today · 2019Review
- Super-Low Dose Lipopolysaccharide Dysregulates Neutrophil Migratory Decision-Making.Frontiers in immunology · 2019Article
- MBiosensors & bioelectronics · 2018Article
- Measuring spontaneous neutrophil motility signatures from a drop of blood using microfluidics.Methods in cell biology · 2018Article
- Neutrophil Chemotaxis in One Droplet of Blood Using Microfluidic Assays.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Linear array of multi-substrate tracts for simultaneous assessment of cell adhesion, migration, and differentiation.BioTechniques · 2017Article
- An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood.Journal of visualized experiments : JoVE · 2017Article
- A Haptotaxis Assay for Neutrophils using Optical Patterning and a High-content Approach.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Improvements in neutrophil chemotaxis assays have advanced our understanding of the mechanisms of neutrophil recruitment; however, traditional methods limit biologic inquiry in important areas. We report a microfluidic technology that enables neutrophil purification and chemotaxis on-chip within minutes, using nanoliters of whole blood, and only requires a micropipette to operate. The low sample volume requirements and novel lid-based method for initiating the gradient of chemoattractant enabled the measurement of human neutrophil migration on a cell monolayer to probe the adherent and migratory states of neutrophils under inflammatory conditions; mouse neutrophil chemotaxis without sacrificing the animal; and both 2D and 3D neutrophil chemotaxis. First, the neutrophil chemotaxis on endothelial cells revealed 2 distinct neutrophil phenotypes, showing that endothelial cell-neutrophil interactions influence neutrophil chemotactic behavior. Second, we validated the mouse neutrophil chemotaxis assay by comparing the adhesion and chemotaxis of neutrophils from chronically inflamed and wild-type mice; we observed significantly higher neutrophil adhesion in blood obtained from chronically inflamed mice. Third, we show that 2D and 3D neutrophil chemotaxis can be directly compared using our technique. These methods allow for new avenues of research while reducing the complexity, time, and sample volume requirements to perform neutrophil chemotaxis assays.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.