ArticlePlant physiology2012
A bifunctional enzyme that has both monoacylglycerol acyltransferase and acyl hydrolase activities.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Early occurrence of caffeoylshikimic acid esterase (CSE) activity in the hornwort Anthoceros agrestis.Planta · 2026Article
- Genetic architecture of the tomato fruit lipidome.PNAS nexus · 2026Article
- Deciphering the role of monoacylglycerol lipases (MAGL) under abiotic stress and lipid metabolism in soybean (Glycine max L.).Plant biotechnology journal · 2025Article
- High resolution QTL mapping and candidate gene mining for seed oil content and fatty acid composition in soybean.BMC plant biology · 2025Article
- Article
- Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism.BMC plant biology · 2024Article
- Genome-wide identification and mining elite allele variation of the Monoacylglycerol lipase (MAGL) gene family in upland cotton (Gossypium hirsutum L.).BMC plant biology · 2024Article
- Characterization of caffeoyl shikimate esterase gene family identifies CsCSE5 as a positive regulator of Podosphaera xanthii and Corynespora cassiicola pathogen resistance in cucumber.Plant cell reports · 2023Article
- Rice lipases: a conundrum in rice bran stabilization: a review on their impact and biotechnological interventions.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Systematic Analysis and Biochemical Characterization of the Caffeoyl Shikimate Esterase Gene Family in Poplar.International journal of molecular sciences · 2021Article
- Functional Characteristics of Caffeoyl Shikimate Esterase inInternational journal of molecular sciences · 2019Article
- Thermodynamic insights into an interaction between ACYL-CoA-BINDING PROTEIN2 and LYSOPHOSPHOLIPASE2 inThe Journal of biological chemistry · 2019Article
- Pseudomonas aeruginosa esterase PA2949, a bacterial homolog of the human membrane esterase ABHD6: expression, purification and crystallization.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Tapetal Expression ofFrontiers in plant science · 2019Article
- Molecular characterization of human ABHD2 as TAG lipase and ester hydrolase.Bioscience reports · 2016Article
- Transcriptome profiling of Camelina sativa to identify genes involved in triacylglycerol biosynthesis and accumulation in the developing seeds.Biotechnology for biofuels · 2016Article
- An annotated database of Arabidopsis mutants of acyl lipid metabolism.Plant cell reports · 2015Article
- Exploring triacylglycerol biosynthetic pathway in developing seeds of Chia (Salvia hispanica L.): a transcriptomic approach.PloS one · 2015Article
- Transcriptional and biochemical responses of monoacylglycerol acyltransferase-mediated oil synthesis and associated senescence-like responses in Nicotiana benthamiana.Frontiers in plant science · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monoacylglycerol acyltransferase (MGAT) catalyzes the synthesis of diacylglycerol, the precursor of triacylglycerol biosynthesis and an important signaling molecule. Here, we describe the isolation and characterization of the peanut (Arachis hypogaea) MGAT gene. The soluble enzyme utilizes invariant histidine-62 and aspartate-67 residues of the acyltransferase motif for its MGAT activity. A sequence analysis revealed the presence of a hydrolase (GXSXG) motif, and enzyme assays revealed the presence of monoacylglycerol (MAG) and lysophosphatidylcholine (LPC) hydrolytic activities, indicating the bifunctional nature of the enzyme. The overexpression of the MGAT gene in yeast (Saccharomyces cerevisiae) caused an increase in triacylglycerol accumulation. Similar to the peanut MGAT, the Arabidopsis (Arabidopsis thaliana) homolog (At1g52760) also exhibited both acyltransferase and hydrolase activities. Interestingly, the yeast homolog lacks the conserved HX(4)D motif, and it is deficient in the acyltransferase function but exhibits MAG and LPC hydrolase activities. This study demonstrates the presence of a soluble MGAT/hydrolase in plants. The predicted three-dimensional homology modeling and substrate docking suggested the presence of two separate substrate (MAG and LPC)-binding sites in a single polypeptide. Our study describes a soluble bifunctional enzyme that has both MGAT and hydrolase functions.
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