ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Projection neurons in lamina III of the rat spinal cord are selectively innervated by local dynorphin-containing excitatory neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Spatial, transcriptomic, and epigenomic analyses link dorsal horn neurons to chronic pain genetic predisposition.Cell reports · 2024Article
- Spatial, transcriptomic and epigenomic analyses link dorsal horn neurons to chronic pain genetic predisposition.bioRxiv : the preprint server for biology · 2024Article
- Paraventricular Hypothalamic Nucleus Upregulates Intraocular Pressure Via Glutamatergic Neurons.Investigative ophthalmology & visual science · 2023Article
- Neuropeptides and small-molecule amine transmitters: cooperative signaling in the nervous system.Cellular and molecular life sciences : CMLS · 2022Review
- Characterisation of deep dorsal horn projection neurons in the spinal cord of the Phox2a::Cre mouse line.Molecular pain · 2022Article
- CRISPR/Cas9-Based Mutagenesis of Histone H3.1 in Spinal Dynorphinergic Neurons Attenuates Thermal Sensitivity in Mice.International journal of molecular sciences · 2022Article
- Morphological investigations of endomorphin-2 and spinoparabrachial projection neurons in the spinal dorsal horn of the rat.Frontiers in neuroanatomy · 2022Article
- Peptide Kappa Opioid Receptor Ligands and Their Potential for Drug Development.Handbook of experimental pharmacology · 2022Review
- The Persistent Pain Transcriptome: Identification of Cells and Molecules Activated by Hyperalgesia.The journal of pain · 2021Article
- Spinal Excitatory Dynorphinergic Interneurons Contribute to Burn Injury-Induced Nociception Mediated by Phosphorylated Histone 3 at Serine 10 in Rodents.International journal of molecular sciences · 2021Article
- Recent advances in our understanding of the organization of dorsal horn neuron populations and their contribution to cutaneous mechanical allodynia.Journal of neural transmission (Vienna, Austria : 1996) · 2020Review
- Spinal Circuits for Touch, Pain, and Itch.Annual review of physiology · 2018Review
- Identifying functional populations among the interneurons in laminae I-III of the spinal dorsal horn.Molecular pain · 2017Review
- Immunostaining for Homer reveals the majority of excitatory synapses in laminae I-III of the mouse spinal dorsal horn.Neuroscience · 2016Article
- The Emerging Role of Spinal Dynorphin in Chronic Pain: A Therapeutic Perspective.Annual review of pharmacology and toxicology · 2016Review
- Article
- Inhibitory Interneurons That Express GFP in the PrP-GFP Mouse Spinal Cord Are Morphologically Heterogeneous, Innervated by Several Classes of Primary Afferent and Include Lamina I Projection Neurons among Their Postsynaptic Targets.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Plasma membrane poration by opioid neuropeptides: a possible mechanism of pathological signal transduction.Cell death & disease · 2015Article
- 3D-printer visualization of neuron models.Frontiers in neuroinformatics · 2015Article
- Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Large projection neurons in lamina III of the rat spinal cord that express the neurokinin 1 receptor are densely innervated by peptidergic primary afferent nociceptors and more sparsely by low-threshold myelinated afferents. However, we know little about their input from other glutamatergic neurons. Here we show that these cells receive numerous contacts from nonprimary boutons that express the vesicular glutamate transporter 2 (VGLUT2), and form asymmetrical synapses on their dendrites and cell bodies. These synapses are significantly smaller than those formed by peptidergic afferents, but provide a substantial proportion of the glutamatergic synapses that the cells receive (over a third of those in laminae I-II and half of those in deeper laminae). Surprisingly, although the dynorphin precursor preprodynorphin (PPD) was only present in 4-7% of VGLUT2 boutons in laminae I-IV, it was found in 58% of the VGLUT2 boutons that contacted these cells. This indicates a highly selective targeting of the lamina III projection cells by glutamatergic neurons that express PPD, and these are likely to correspond to local neurons (interneurons and possibly projection cells). Since many PPD-expressing dorsal horn neurons respond to noxious stimulation, this suggests that the lamina III projection cells receive powerful monosynaptic and polysynaptic nociceptive input. Excitatory interneurons in the dorsal horn have been shown to possess I(A) currents, which limit their excitability and can underlie a form of activity-dependent intrinsic plasticity. It is therefore likely that polysynaptic inputs to the lamina III projection neurons are recruited during the development of chronic pain states.
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