ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Microglia proliferation is controlled by P2X7 receptors in a Pannexin-1-independent manner during early embryonic spinal cord invasion.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Methodological guidelines for P2X receptor assays and data interpretation.Cell death & disease · 2026Review
- P2X7 receptor-dependent microglia-astrocyte coupling in Alzheimer's disease: from eATP sensing to synaptic and proteostatic failure.Frontiers in aging neuroscience · 2026Review
- Dual role for pannexin 1 at synapses: regulating functional and morphological plasticity.The Journal of physiology · 2025Review
- The Multifaceted Role of P2X7R in Microglia and Astrocytes.Neurochemical research · 2025Review
- Harmonizing TUNEL with multiplexed iterative immunofluorescence enriches spatial contextualization of cell death.Cell reports methods · 2025Article
- New insights into pathogenisis and therapies of P2X7R in Parkinson's disease.NPJ Parkinson's disease · 2025Review
- P2X7 receptors and pannexin1 hemichannels shape presynaptic transmission.Purinergic signalling · 2024Review
- Origin and Emergence of Microglia in the CNS-An Interesting (Hi)story of an Eccentric Cell.Current issues in molecular biology · 2023Review
- Astrocyte and neuronal Panx1 support long-term reference memory in mice.bioRxiv : the preprint server for biology · 2023Article
- Characterization of the Novel P2X7 Receptor Radioligand [ACS chemical neuroscience · 2023Article
- Inherent P2X7 Receptors Regulate Macrophage Functions during Inflammatory Diseases.International journal of molecular sciences · 2021Review
- Neonatal Seizures and Purinergic Signalling.International journal of molecular sciences · 2020Review
- Regulation of Microglial Functions by Purinergic Mechanisms in the Healthy and Diseased CNS.Cells · 2020Review
- The Role of P2X7 Receptor in Alzheimer's Disease.Frontiers in molecular neuroscience · 2020Review
- Microglia: Lifelong patrolling immune cells of the brain.Progress in neurobiology · 2019Review
- Pleiotropic Roles of P2X7 in the Central Nervous System.Frontiers in cellular neuroscience · 2019Review
- P2X7 as a scavenger receptor for innate phagocytosis in the brain.British journal of pharmacology · 2018Review
- The role of connexin and pannexin containing channels in the innate and acquired immune response.Biochimica et biophysica acta. Biomembranes · 2018Review
- Interactions of Pannexin1 channels with purinergic and NMDA receptor channels.Biochimica et biophysica acta. Biomembranes · 2018Review
- The role of microglial P2X7: modulation of cell death and cytokine release.Journal of neuroinflammation · 2017Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microglia are known to invade the mammalian spinal cord (SC) at an early embryonic stage. While the mechanisms underlying this early colonization of the nervous system are still unknown, we recently found that it is associated, at least partially, with the ability of microglia to proliferate at the onset of motoneuron developmental cell death and of synaptogenesis in mouse embryo (E13.5). In vitro studies have shown that the proliferation and activation of adult microglia can be influenced by the purinergic ionotropic receptor P2X7 via a coupling with Pannexin-1. By performing patch-clamp recordings in situ using a whole-mouse embryonic SC preparation, we show here that embryonic microglia already express functional P2X7R. P2X7R activation evoked a biphasic current in embryonic microglia, which is supposed to reflect large plasma membrane pore opening. However, although embryonic microglia express pannexin-1, this biphasic current was still recorded in microglia of pannexin-1 knock-out embryos, indicating that it rather reflected P2X7R intrinsic pore dilatation. More important, we found that proliferation of embryonic SC microglia, but not their activation state, depends almost entirely on P2X7R by comparing wild-type and P2X7R-/- embryos. Absence of P2X7R led also to a decrease in microglia density. Pannexin-1-/- embryos did not exhibit any difference in microglial proliferation, showing that the control of embryonic microglial proliferation by P2X7R does not depend on pannexin-1 expression. These results reveal a developmental role of P2X7R by controlling embryonic SC microglia proliferation at a critical developmental state in the SC of mouse embryos.
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