ArticleWorld journal of microbiology & biotechnology2013
Engineered Bacillus subtilis 168 produces L-malate by heterologous biosynthesis pathway construction and lactate dehydrogenase deletion.
Article in World journal of microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 33 citations in OpenAlex.
- Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression inMicroorganisms · 2023Article
- Recent advances in the production of malic acid by native fungi and engineered microbes.World journal of microbiology & biotechnology · 2023Review
- Recent advances in producing food additive L-malate: Chassis, substrate, pathway, fermentation regulation and application.Microbial biotechnology · 2023Review
- Enzymatic Characterization and Coenzyme Specificity Conversion of a Novel Dimeric Malate Dehydrogenase from Bacillus subtilis.The protein journal · 2023Article
- Microbial Biosynthesis of L-Malic Acid and Related Metabolic Engineering Strategies: Advances and Prospects.Frontiers in bioengineering and biotechnology · 2021Review
- Current development in genetic engineering strategies of Bacillus species.Microbial cell factories · 2014Review
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
In the present work, Bacillus subtilis was engineered to produce L-malate. Initially, the study revealed that the slight fumarase activity under anaerobic conditions is extremely favourable for L-malate one-step fermentation accumulation. Subsequently, an efficient heterologous biosynthesis pathway formed by Escherichia coli phosphoenolpyruvate carboxylase and Saccharomyces cerevisiae malate dehydrogenase was introduced into B. subtilis, which led to 6.04 ± 0.19 mM L-malate production. Finally, the L-malate production was increased 1.5-fold to 9.18 ± 0.22 mM by the deletion of lactate dehydrogenase. Under two-stage fermentation conditions, the engineered B. subtilis produced up to 15.65 ± 0.13 mM L-malate, which was 86.3 % higher than that under anaerobic fermentation conditions. Though the L-malate production by the recombinant was low, this is the first attempt to produce L-malate in engineered B. subtilis and paves the way for further improving L-malate production in B. subtilis.
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