ArticleAmerican journal of physiology. Cell physiology2012
The Drosophila NKCC Ncc69 is required for normal renal tubule function.
Article in American journal of physiology. Cell physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 65 citations in OpenAlex.
- Molecular and neural mechanisms of sodium sensation.Genes & genomics · 2026Review
- Dynamics of release and activity of select neuropeptides post-bloodmeal in the female mosquito, Aedes aegypti.The Journal of experimental biology · 2025Article
- Function and regulation of the insect NaCCC2 sodium transport proteins.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2024Article
- Circadian Rhythm Regulation by Pacemaker Neuron Chloride Oscillation in Flies.Physiology (Bethesda, Md.) · 2024Review
- Differential amino acid transporter expression in adultmicroPublication biology · 2024Article
- The V-type HProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Unanticipated domain requirements for Drosophila Wnk kinase in vivo.PLoS genetics · 2023Article
- Review
- Chloride oscillation in pacemaker neurons regulates circadian rhythms through a chloride-sensing WNK kinase signaling cascade.Current biology : CB · 2022Article
- Updates on ion and water transport by the Malpighian tubule.Current opinion in insect science · 2021Review
- WNKs are potassium-sensitive kinases.American journal of physiology. Cell physiology · 2021Article
- A neuroendocrine pathway modulating osmotic stress in Drosophila.PLoS genetics · 2021Article
- Fluid Secretion by Malpighian Tubules ofFrontiers in endocrinology · 2021Review
- The septate junction protein Mesh is required for epithelial morphogenesis, ion transport, and paracellular permeability in theAmerican journal of physiology. Cell physiology · 2020Article
- CAPA neuropeptides and their receptor form an anti-diuretic hormone signaling system in the human disease vector, Aedes aegypti.Scientific reports · 2020Article
- Physiology, Development, and Disease Modeling in theGenetics · 2020Review
- Specialized stellate cells offer a privileged route for rapid water flux inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- The Drosophila Malpighian tubule as a model for mammalian tubule function.Current opinion in nephrology and hypertension · 2019Review
- Heterologous Expression ofInsects · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Epithelial ion transport is essential to renal homeostatic function, and it is dysregulated in several diseases, such as hypertension. An understanding of the insect renal (Malpighian) tubule yields insights into conserved epithelial ion transport processes in higher organisms and also has implications for the control of insect infectious disease vectors. Here, we examine the role of the Na(+)-K(+)-2Cl(-) (NKCC) cotransporter Ncc69 in Drosophila tubule function. Ncc69 mutant tubules have decreased rates of fluid secretion and K(+) flux, and these phenotypes were rescued by expression of wild-type Ncc69 in the principal cells of the tubule. Na(+) flux was unaltered in Ncc69 mutants, suggesting Na(+) recycling across the basolateral membrane. In unstimulated tubules, the principal role of the Na(+)-K(+)-ATPase is to generate a favorable electrochemical gradient for Ncc69 activity: while the Na(+)-K(+)-ATPase inhibitor ouabain decreased K(+) flux in wild-type tubules, it had no effect in Ncc69 mutant tubules. However, in the presence of cAMP, which stimulates diuresis, additional Na(+)-K(+)-ATPase-dependent K(+) transport pathways are recruited. In studying the effects of capa-1 on wild-type and Ncc69 mutant tubules, we found a novel antidiuretic role for this hormone that is dependent on intact Ncc69, as it was abolished in Ncc69 mutant tubules. Thus, Ncc69 plays an important role in transepithelial ion and fluid transport in the fly renal tubule and is a target for regulation in antidiuretic states.
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