ArticleStem cells and development2013
PKH26 can transfer to host cells in vitro and vivo.
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Advances in Tracing Techniques: Mapping the Trajectory of Mesenchymal Stem-Cell-Derived Extracellular Vesicles.Chemical & biomedical imaging · 2025Review
- Anti-Cancer and Pro-Immune Effects of Lauric Acid on Colorectal Cancer Cells.International journal of molecular sciences · 2025Article
- CXCL10 is a crucial chemoattractant for efficient intranasal delivery of mesenchymal stem cells to the neonatal hypoxic-ischemic brain.Stem cell research & therapy · 2024Article
- Tracking of Stem Cells in Chronic Liver Diseases: Current Trends and Developments.Stem cell reviews and reports · 2024Review
- Role of adipose-derived stem cells in healing surgically induced trauma of the rat's tunica albuginea.Sexual medicine · 2023Article
- Long-term and non-invasiveWorld journal of hepatology · 2022Article
- Tracking of Extracellular Vesicles' Biodistribution: New Methods and Approaches.International journal of molecular sciences · 2022Review
- A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles.Scientific reports · 2022Article
- Therapeutic role of adipose tissue-derived stem cells versus microvesicles in a rat model of cerebellar injury.Journal of cellular and molecular medicine · 2022Article
- Novel dual-fluorescent flow cytometric approach for quantification of macrophages infected withParasitology · 2022Article
- Large Animal Models for Investigating Cell Therapies of Stress Urinary Incontinence.International journal of molecular sciences · 2021Review
- Article
- Bone marrow-derived mesenchymal stem cells attenuate pulmonary inflammation and lung damage caused by highly pathogenic avian influenza A/H5N1 virus in BALB/c mice.BMC infectious diseases · 2020Article
- Delivery of a model lipophilic membrane cargo to bone marrow via cell-derived microparticles.Journal of controlled release : official journal of the Controlled Release Society · 2020Article
- Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and TScience advances · 2020Article
- Acellular bioscaffolds redirect cardiac fibroblasts and promote functional tissue repair in rodents and humans with myocardial injury.Scientific reports · 2020Observational
- Chemotherapy resistance and stemness in mitotically quiescent human breast cancer cells identified by fluorescent dye retention.Clinical & experimental metastasis · 2018Article
- Non-invasive imaging reveals conditions that impact distribution and persistence of cells after in vivo administration.Stem cell research & therapy · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fluorescent dye, PKH26, which mainly binds to the cell membrane, has been used as the cell tracer to locate the transplanted cells in host for a long time. However, there was no detailed report that whether the PKH26 dye was specific to the transplanted cells. Therefore, the aim of this article is to explore the effect of cells debris as the cracking cells from the PKH26-labeled adipose-derived stem cells (ADSCs) on the cells in vitro and the host in vivo. After we tested the proliferation and toxicity of PKH26 to the ADSCs by the Cell Count-8 kit and alamar blue assay, we constructed 2 models, coculturing lots of PKH26-labeled cell debris with the unlabeled ADSCs in vitro and injecting via the tail vein in rat, to evaluate the specificity of the PKH26 dye. The result indicated that the PKH26 didn't inhibit the proliferation and had no toxicity to the ADSCs compared with the unlabeled ADSCs, but the cell debris cracking from PKH26-labeled transplanted cells can cause the unlabeled cells to emit red fluorescence in vitro and also lead the tissues displaying red fluorescence in vivo. We can conclude that the PKH26 dye, used as a cell tracer for a long time, was not an ideal cell tracer.
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