ArticleAnalytical chemistry2012
Chaperone probes and bead-based enhancement to improve the direct detection of mRNA using silicon photonic sensor arrays.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Biofunctionalization of Multiplexed Silicon Photonic Biosensors.Biosensors · 2022Review
- High resolution biosensor to test the capping level and integrity of mRNAs.Nucleic acids research · 2020Article
- Multiplexed microRNA Expression Profiling by Combined Asymmetric PCR and Label-Free Detection using Silicon Photonic Sensor Arrays.Analytical methods : advancing methods and applications · 2018Article
- Combining asymmetric PCR-based enzymatic amplification with silicon photonic microring resonators for the detection of lncRNAs from low input human RNA samples.The Analyst · 2018Article
- Multiplexed silicon photonic sensor arrays enable facile characterization of coagulation protein binding to nanodiscs with variable lipid content.The Journal of biological chemistry · 2017Article
- A Guide to Quantitative Biomarker Assay Development using Whispering Gallery Mode Biosensors.Current protocols in chemical biology · 2017Article
- Targeted Treatment for Bacterial Infections: Prospects for Pathogen-Specific Antibiotics Coupled with Rapid Diagnostics.Tetrahedron · 2016Article
- Applications of Optical Microcavity Resonators in Analytical Chemistry.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Article
- Emerging Biosensing Approaches for microRNA Analysis.Analytical chemistry · 2016Review
- Next generation ligand binding assays-review of emerging real-time measurement technologies.The AAPS journal · 2014Review
- Biomolecular analysis with microring resonators: applications in multiplexed diagnostics and interaction screening.Current opinion in chemical biology · 2013Review
- Interfacing lipid bilayer nanodiscs and silicon photonic sensor arrays for multiplexed protein-lipid and protein-membrane protein interaction screening.Analytical chemistry · 2013Article
- Single domain antibodies for the detection of ricin using silicon photonic microring resonator arrays.Analytical chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Herein, we describe the utility of chaperone probes and a bead-based signal enhancement strategy for the analysis of full length mRNA transcripts using arrays of silicon photonic microring resonators. Changes in the local refractive index near microring sensors associated with biomolecular binding events are transduced as a shift in the resonant wavelength supported by the cavity, enabling the sensitive analysis of numerous analytes of interest. We employ the sensing platform for both the direct and bead-enhanced detection of three different mRNA transcripts, achieving a dynamic range spanning over 4 orders of magnitude and demonstrating expression profiling capabilities in total RNA extracts from the HL-60 cell line. Small, dual-use DNA chaperone molecules were developed and found to both enhance the binding kinetics of mRNA transcripts by disrupting complex secondary structure and serve as sequence-specific linkers for subsequent bead amplification. Importantly, this approach does not require amplification of the mRNA transcript, thereby allowing for simplified analyses that do not require expensive enzymatic reagents or temperature ramping capabilities associated with RT-PCR-based methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.