ArticlePloS one2012
Phosphoproteome of human glioblastoma initiating cells reveals novel signaling regulators encoded by the transcriptome.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Real-time search-assisted multiplexed quantitative proteomics reveals system-wide cryptic translation initiation in human cancer cells.Genome biology · 2026Article
- Nondomain biopolymers: Flexible molecular strategies to acquire biological functions.Genes to cells : devoted to molecular & cellular mechanisms · 2023Review
- Glioblastoma stem cells express non-canonical proteins and exclusive mesenchymal-like or non-mesenchymal-like protein signatures.Molecular oncology · 2023Article
- Phosphoproteomic Analysis Defines BABAM1 as mTORC2 Downstream Effector Promoting DNA Damage Response in Glioblastoma Cells.Journal of proteome research · 2022Article
- Article
- Phase separation in epigenetics and cancer stem cells.Frontiers in oncology · 2022Review
- System-Wide Analysis of Protein Acetylation and Ubiquitination Reveals a Diversified Regulation in Human Cancer Cells.Biomolecules · 2020Article
- Myosin-IIA heavy chain phosphorylation on S1943 regulates tumor metastasis.Experimental cell research · 2018Article
- Revisiting CDK Inhibitors for Treatment of Glioblastoma Multiforme.Drugs in R&D · 2017Review
- Understanding cell signaling in cancer stem cells for targeted therapy - can phosphoproteomics help to reveal the secrets?Cell communication and signaling : CCS · 2017Review
- Integrative Network Analysis Combined with Quantitative Phosphoproteomics Reveals Transforming Growth Factor-beta Receptor type-2 (TGFBR2) as a Novel Regulator of Glioblastoma Stem Cell Properties.Molecular & cellular proteomics : MCP · 2016Article
- Long noncoding RNA UPAT promotes colon tumorigenesis by inhibiting degradation of UHRF1.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Emerging Proteomic Technologies Provide Enormous and Underutilized Potential for Brain Cancer Research.Molecular & cellular proteomics : MCP · 2016Review
- Function of the Herpes Simplex Virus 1 Small Capsid Protein VP26 Is Regulated by Phosphorylation at a Specific Site.Journal of virology · 2015Article
- Kinomic exploration of temozolomide and radiation resistance in Glioblastoma multiforme xenolines.Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology · 2014Article
- Toward a systems-level view of dynamic phosphorylation networks.Frontiers in genetics · 2014Review
- Hypoxia-inducible factor 1 regulation through cross talk between mTOR and MT1-MMP.Molecular and cellular biology · 2014Article
- Protein kinase D2 regulates migration and invasion of U87MG glioblastoma cells in vitro.Experimental cell research · 2013Article
- The cancer stem cell marker CD133 interacts with plakoglobin and controls desmoglein-2 protein levels.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGlioblastoma is one of the most aggressive tumors with poor prognosis. Although various studies have been performed so far, there are not effective treatments for patients with glioblastoma. METHODOLOGY/PRINCIPAL
findingsIn order to systematically elucidate the aberrant signaling machinery activated in this malignant brain tumor, we investigated phosphoproteome dynamics of glioblastoma initiating cells using high-resolution nanoflow LC-MS/MS system in combination with SILAC technology. Through phosphopeptide enrichment by titanium dioxide beads, a total of 6,073 phosphopeptides from 2,282 phosphorylated proteins were identified based on the two peptide fragmentation methodologies of collision induced dissociation and higher-energy C-trap dissociation. The SILAC-based quantification described 516 up-regulated and 275 down-regulated phosphorylation sites upon epidermal growth factor stimulation, including the comprehensive status of the phosphorylation sites on stem cell markers such as nestin. Very intriguingly, our in-depth phosphoproteome analysis led to identification of novel phosphorylated molecules encoded by the undefined sequence regions of the human transcripts, one of which was regulated upon external stimulation in human glioblastoma initiating cells. CONCLUSIONS/SIGNIFICANCE: Our result unveils an expanded diversity of the regulatory phosphoproteome defined by the human transcriptome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.