ArticlePloS one2012
Enhanced UV resistance and improved killing of malaria mosquitoes by photolyase transgenic entomopathogenic fungi.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Development of Mycoinsecticides: Advances in Formulation, Regulatory Challenges and Market Trends for Entomopathogenic Fungi.Journal of fungi (Basel, Switzerland) · 2025Review
- Engineered Metarhizium fungi produce longifolene to attract and kill mosquitoes.Nature microbiology · 2025Article
- Larvicidal activity of Trichoderma atroviride (Hypocreales: Hypocreaceae) against Aedes albopictus (Diptera: Culicidae).Journal of medical entomology · 2025Article
- Mr-lac3 and Mr-lcc2 inJournal of fungi (Basel, Switzerland) · 2025Article
- MaAzaR, a ZnJournal of fungi (Basel, Switzerland) · 2024Article
- A recombinant fungal photolyase autonomously enters human cell nuclei to fix UV-induced DNA lesions.Biotechnology letters · 2024Article
- Recent Advancements in Pathogenic Mechanisms, Applications and Strategies for Entomopathogenic Fungi in Mosquito Biocontrol.Journal of fungi (Basel, Switzerland) · 2023Review
- The Potential of a New Beauveria bassiana Isolate for Mosquito Larval Control.Journal of medical entomology · 2023Article
- Physiological and Molecular Response Modifications by Ultraviolet-C Radiation inInternational journal of molecular sciences · 2022Article
- Current developments in the resistance, quality, and production of entomopathogenic fungi.World journal of microbiology & biotechnology · 2022Review
- Enhancing the Biocontrol Potential of the Entomopathogenic Fungus in Multiple Respects via the Overexpression of a Transcription Factor GeneJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Article
- Combining Transcriptomics and Proteomics Reveals Potential Post-transcriptional Control of Gene Expression After Light Exposure inG3 (Bethesda, Md.) · 2019Article
- Two Photolyases Repair Distinct DNA Lesions and Reactivate UVB-Inactivated Conidia of an Insect Mycopathogen under Visible Light.Applied and environmental microbiology · 2019Article
- Duplication of a Pks gene cluster and subsequent functional diversification facilitate environmental adaptation in Metarhizium species.PLoS genetics · 2018Article
- The Rab GTPase activating protein Gyp2 contributes to UV stress tolerance in Metarhizium acridum.World journal of microbiology & biotechnology · 2018Article
- The Insect Pathogens.Microbiology spectrum · 2017Review
- Stress response signaling and virulence: insights from entomopathogenic fungi.Current genetics · 2015Review
- Molecular and physiological effects of environmental UV radiation on fungal conidia.Current genetics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The low survival of microbial pest control agents exposed to UV is the major environmental factor limiting their effectiveness. Using gene disruption we demonstrated that the insect pathogenic fungus Metarhizium robertsii uses photolyases to remove UV-induced cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) photoproducts [(6-4)PPs] from its DNA. However, this photorepair is insufficient to fix CPD lesions and prevent the loss of viability caused by seven hours of solar radiation. Expression of a highly efficient archaeal (Halobacterium salinarum) CPD photolyase increased photorepair >30-fold in both M. robertsii and Beauveria bassiana. Consequently, transgenic strains were much more resistant to sunlight and retained virulence against the malaria vector Anopheles gambiae. In the field this will translate into much more efficient pest control over a longer time period. Conversely, our data shows that deleting native photolyase genes will strictly contain M. robertsii to areas protected from sunlight, alleviating safety concerns that transgenic hypervirulent Metarhizium spp will spread from mosquito traps or houses. The precision and malleability of the native and transgenic photolyases allows design of multiple pathogens with different strategies based on the environments in which they will be used.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.