ArticlePloS one2012
Diffusion barriers constrain receptors at synapses.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- How energy determines spatial localisation and copy number of molecules in neurons.Nature communications · 2025Article
- Chronic benzodiazepine treatment triggers gephyrin scaffold destabilization and GABAFrontiers in cellular neuroscience · 2025Article
- LRRTM2 controls presynapse nano-organization and AMPA receptor sub-positioning through Neurexin-binding interface.Nature communications · 2024Article
- Activity-dependent diffusion trapping of AMPA receptors as a key step for expression of early LTP.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- NKCC1 and KCC2 Chloride Transporters Have Different Membrane Dynamics on the Surface of Hippocampal Neurons.Cells · 2023Article
- Article
- Conformational state-dependent regulation of GABAiScience · 2022Article
- Characterization of the Functional Cross-Talk between Surface GABAInternational journal of molecular sciences · 2021Article
- Molecular Crowding and Diffusion-Capture in Synapses.iScience · 2020Article
- AMPA receptors in the synapse turnover by monomer diffusion.Nature communications · 2019Article
- Alterations in GABAFrontiers in cellular neuroscience · 2019Article
- Neurobiology and Therapeutic Potential of α5-GABA Type A Receptors.Frontiers in molecular neuroscience · 2019Article
- Diazepam Accelerates GABAFrontiers in cellular neuroscience · 2019Article
- Neuronal Receptors Display Cytoskeleton-Independent Directed Motion on the Plasma Membrane.iScience · 2018Article
- Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses.Nanoscale · 2018Article
- Self-crowding of AMPA receptors in the excitatory postsynaptic density can effectuate anomalous receptor sub-diffusion.PLoS computational biology · 2018Article
- γ2 GABAFrontiers in cellular neuroscience · 2018Review
- Depolarizing, inhibitory GABA type A receptor activity regulates GABAergic synapse plasticity via ERK and BDNF signaling.Neuropharmacology · 2018Article
- A Simple and Powerful Analysis of Lateral Subdiffusion Using Single Particle Tracking.Biophysical journal · 2017Article
- GABANature communications · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The flux of neurotransmitter receptors in and out of synapses depends on receptor interaction with scaffolding molecules. However, the crowd of transmembrane proteins and the rich cytoskeletal environment may constitute obstacles to the diffusion of receptors within the synapse. To address this question, we studied the membrane diffusion of the γ-aminobutyric acid type A receptor (GABA(A)R) subunits clustered (γ2) or not (α5) at inhibitory synapses in rat hippocampal dissociated neurons. Relative to the extrasynaptic region, γ2 and α5 showed reduced diffusion and increased confinement at both inhibitory and excitatory synapses but they dwelled for a short time at excitatory synapses. In contrast, γ2 was ~3-fold more confined and dwelled ~3-fold longer in inhibitory synapses than α5, indicating faster synaptic escape of α5. Furthermore, using a gephyrin dominant-negative approach, we showed that the increased residency time of γ2 at inhibitory synapses was due to receptor-scaffold interactions. As shown for GABA(A)R, the excitatory glutamate receptor 2 subunit (GluA2) of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) had lower mobility in both excitatory and inhibitory synapses but a higher residency time at excitatory synapses. Therefore barriers impose significant diffusion constraints onto receptors at synapses where they accumulate or not. Our data further reveal that the confinement and the dwell time but not the diffusion coefficient report on the synapse specific sorting, trapping and accumulation of receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.