ArticlePloS one2012
Comparative transcriptomics of the saprobic and parasitic growth phases in Coccidioides spp.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 80 citations in OpenAlex.
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- Transcriptomic characterization of Coccidioides morphological states using RiboMarker-enhanced RNA sequencing.G3 (Bethesda, Md.) · 2026Article
- Hybrid histidine kinases and antifungal warfare in thermal dimorphic fungi.Antimicrobial agents and chemotherapy · 2026Review
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- Comparison of Patients With Coccidioidomycosis in Arizona Versus California: A Commercial Health Insurance Claims Analysis.Open forum infectious diseases · 2025Article
- Transcriptomic atlas throughout Coccidioides development reveals key phase-enriched transcripts of this important fungal pathogen.PLoS biology · 2025Article
- Fungal Metabolomics: A Comprehensive Approach to Understanding Pathogenesis in Humans and Identifying Potential Therapeutics.Journal of fungi (Basel, Switzerland) · 2025Review
- Transcriptomic atlas of the morphologic development of the fungal pathogenbioRxiv : the preprint server for biology · 2024Article
- Diagnosis, Treatment, and Outcome of Coccidioidal Osseous Infections: A Systematic Review.Journal of fungi (Basel, Switzerland) · 2024Review
- Evaluation of Different Gene Prediction Tools inJournal of fungi (Basel, Switzerland) · 2023Article
- Coccidioidomycosis Granulomas Informed by Other Diseases: Advancements, Gaps, and Challenges.Journal of fungi (Basel, Switzerland) · 2023Review
- Spinal Infections Caused byMaedica · 2023Article
- Genome Organization and Copy-Number Variation Reveal Clues to Virulence Evolution inJournal of fungi (Basel, Switzerland) · 2022Article
- A dissemination-prone morphotype enhances extrapulmonary organ entry by Cryptococcus neoformans.Cell host & microbe · 2022Article
- Genomic Data Mining Reveals Abundant Uncharacterized Transporters inJournal of fungi (Basel, Switzerland) · 2022Article
- Coccidioides Species: A Review of Basic Research: 2022.Journal of fungi (Basel, Switzerland) · 2022Review
- Characterization of the Growth and Morphology of a BSL-2Journal of fungi (Basel, Switzerland) · 2022Article
- A chromosomal-level reference genome of the widely utilized Coccidioides posadasii laboratory strain "Silveira".G3 (Bethesda, Md.) · 2022Article
- The WOPR family protein Ryp1 is a key regulator of gene expression, development, and virulence in the thermally dimorphic fungal pathogen Coccidioides posadasii.PLoS pathogens · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Coccidioides immitis and C. posadasii, the causative agents of coccidioidomycosis, are dimorphic fungal pathogens, which grow as hyphae in the saprobic phase in the environment and as spherules in the parasitic phase in the mammalian host. In this study, we use comparative transcriptomics to identify gene expression differences between the saprobic and parasitic growth phases. We prepared Illumina mRNA sequencing libraries for saprobic-phase hyphae and parasitic-phase spherules in vitro for C. immitis isolate RS and C. posadasii isolate C735 in biological triplicate. Of 9,910 total predicted genes in Coccidioides, we observed 1,298 genes up-regulated in the saprobic phase of both C. immitis and C. posadasii and 1,880 genes up-regulated in the parasitic phase of both species. Comparing the saprobic and parasitic growth phases, we observed considerable differential expression of cell surface-associated genes, particularly chitin-related genes. We also observed differential expression of several virulence factors previously identified in Coccidioides and other dimorphic fungal pathogens. These included alpha (1,3) glucan synthase, SOWgp, and several genes in the urease pathway. Furthermore, we observed differential expression in many genes predicted to be under positive selection in two recent Coccidioides comparative genomics studies. These results highlight a number of genes that may be crucial to dimorphic phase-switching and virulence in Coccidioides. These observations will impact priorities for future genetics-based studies in Coccidioides and provide context for studies in other fungal pathogens.
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