ArticlePloS one2012
Enhanced keratinocyte proliferation and migration in co-culture with fibroblasts.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it, 125 citations in OpenAlex.
- Interplay Between Keratinocytes and Fibroblasts: A Systematic Review Providing a New Angle for Understanding Skin Fibrotic Disorders.Frontiers in immunology · 2020Pooled it
- Culture and gene expression profiling of Schwann cells from human sural nerve.Communications biology · 2026Article
- 3D Bioprinted Alginate-Gelatin-Collagen Based Hydrogels Laden With Fibroblast and Keratinocyte Cells Show Potential for Regeneration of Injured Skin.Macromolecular bioscience · 2026Article
- Skin Cells' Protection Against UVA-Induced Changes in Co-Cultured Keratinocytes-Fibroblasts' Proteome and Released Signaling Proteins by 3-International journal of molecular sciences · 2026Article
- Article
- A stretch-responsive fibroblast program promotes epidermal stem cell self-renewal during skin expansion.Nature communications · 2026Article
- Effects of leptin on growth factor expression in human keratinocytes.Journal of molecular histology · 2026Article
- Exploring Compositions of Cell-Based Therapies for Diabetic Foot Ulcer Treatment: A Review of Clinical Studies.Stem cell reviews and reports · 2026Review
- The Effects of PLGA Nanoparticles Containing Different Growth Factors on Neural Stem Cell Differentiation and Their Transition Efficiency After Targeting With TRF.CNS neuroscience & therapeutics · 2025Article
- Photodynamically tunable ROS-generating hydrogels for accelerated tissue regeneration.Bioactive materials · 2025Article
- Synthesis of aACS applied bio materials · 2025Article
- Article
- Effect of placental mesenchymal stem cells on promoting the healing of chronic burn wounds.Heliyon · 2024Article
- Exploring Skin Wound Healing Models and the Impact of Natural Lipids on the Healing Process.International journal of molecular sciences · 2024Review
- Article
- Self-Assembled Fibrinogen Scaffolds Support Cocultivation of Human Dermal Fibroblasts and HaCaT Keratinocytes.ACS omega · 2023Article
- Novel Electrospun Polycaprolactone/Calcium Alginate Scaffolds for Skin Tissue Engineering.Materials (Basel, Switzerland) · 2022Article
- Evaluation of native and non-native biomaterials for engineering human skin tissue.Bioengineering & translational medicine · 2022Article
- Tapping basement membrane motifs: Oral junctional epithelium for surface-mediated soft tissue attachment to prevent failure of percutaneous devices.Acta biomaterialia · 2022Article
- Sustainable phyto-fabrication of silver nanoparticles using Gmelina arborea exhibit antimicrobial and biofilm inhibition activity.Scientific reports · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wound healing is primarily controlled by the proliferation and migration of keratinocytes and fibroblasts as well as the complex interactions between these two cell types. To investigate the interactions between keratinocytes and fibroblasts and the effects of direct cell-to-cell contact on the proliferation and migration of keratinocytes, keratinocytes and fibroblasts were stained with different fluorescence dyes and co-cultured with or without transwells. During the early stage (first 5 days) of the culture, the keratinocytes in contact with fibroblasts proliferated significantly faster than those not in contact with fibroblasts, but in the late stage (11(th) to 15(th) day), keratinocyte growth slowed down in all cultures unless EGF was added. In addition, keratinocyte migration was enhanced in co-cultures with fibroblasts in direct contact, but not in the transwells. Furthermore, the effects of the fibroblasts on keratinocyte migration and growth at early culture stage correlated with heparin-binding EGF-like growth factor (HB-EGF), IL-1α and TGF-β1 levels in the cultures where the cells were grown in direct contact. These effects were inhibited by anti-HB-EGF, anti-IL-1α and anti-TGF-β1 antibodies and anti-HB-EGF showed the greatest inhibition. Co-culture of keratinocytes and IL-1α and TGF-β1 siRNA-transfected fibroblasts exhibited a significant reduction in HB-EGF production and keratinocyte proliferation. These results suggest that contact with fibroblasts stimulates the migration and proliferation of keratinocytes during wound healing, and that HB-EGF plays a central role in this process and can be up-regulated by IL-1α and TGF-β1, which also regulate keratinocyte proliferation differently during the early and late stage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.