ArticleLab on a chip2012
A mini-microscope for in situ monitoring of cells.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Automated Open-Hardware Multiwell Imaging Station for Microorganisms Observation.Micromachines · 2022Article
- Picroscope: low-cost system for simultaneous longitudinal biological imaging.Communications biology · 2021Article
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- Miniaturized modular-array fluorescence microscopy.Biomedical optics express · 2020Article
- Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies?Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Monitoring of Microphysiological Systems: Integrating Sensors and Real-Time Data Analysis toward Autonomous Decision-Making.ACS sensors · 2019Article
- A miniaturized optical tomography platform for volumetric imaging of engineered living systems.Lab on a chip · 2019Article
- Benchmarking Smartphone Fluorescence-Based Microscopy with DNA Origami Nanobeads: Reducing the Gap toward Single-Molecule Sensitivity.ACS omega · 2019Article
- Article
- MBiosensors & bioelectronics · 2018Article
- Microphysiologic systems in female reproductive biology.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Expansion Mini-Microscopy: An Enabling Alternative in Point-of-Care Diagnostics.Current opinion in biomedical engineering · 2017Article
- An automated system for high-throughput generation and optimization of microdroplets.Biomicrofluidics · 2016Article
- Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling.Drug discovery today · 2016Review
- Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications.Scientific reports · 2016Article
- Google Glass-Directed Monitoring and Control of Microfluidic Biosensors and Actuators.Scientific reports · 2016Article
- Compact Wireless Microscope for In-Situ Time Course Study of Large Scale Cell Dynamics within an Incubator.Scientific reports · 2015Article
- Aligned carbon nanotube-based flexible gel substrates for engineering bio-hybrid tissue actuators.Advanced functional materials · 2015Article
- Quantification of plant chlorophyll content using Google Glass.Lab on a chip · 2015Article
- A cost-effective fluorescence mini-microscope for biomedical applications.Lab on a chip · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
A mini-microscope was developed for in situ monitoring of cells by modifying off-the-shelf components of a commercial webcam. The mini-microscope consists of a CMOS imaging module, a small plastic lens and a white LED illumination source. The CMOS imaging module was connected to a laptop computer through a USB port for image acquisition and analysis. Due to its compact size, 8 × 10 × 9 cm, the present microscope is portable and can easily fit inside a conventional incubator, and enables real-time monitoring of cellular behaviour. Moreover, the mini-microscope can be used for imaging cells in conventional cell culture flasks, such as Petri dishes and multi-well plates. To demonstrate the operation of the mini-microscope, we monitored the cellular migration of mouse 3T3 fibroblasts in a scratch assay in medium containing three different concentrations of fetal bovine serum (5, 10, and 20%) and demonstrated differential responses depending on serum levels. In addition, we seeded embryonic stem cells inside poly(ethylene glycol) microwells and monitored the formation of stem cell aggregates in real time using the mini-microscope. Furthermore, we also combined a lab-on-a-chip microfluidic device for microdroplet generation and analysis with the mini-microscope and observed the formation of droplets under different flow conditions. Given its cost effectiveness, robust imaging and portability, the presented platform may be useful for a range of applications for real-time cellular imaging using lab-on-a-chip devices at low cost.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.