ArticleJournal of industrial microbiology & biotechnology2012
Co-fermentation of xylose and cellobiose by an engineered Saccharomyces cerevisiae.
Article in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Engineering Saccharomyces Cerevisiae With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production.Biotechnology journal · 2025Article
- Recent progress in engineering yeast producers of cellulosic ethanol.FEMS yeast research · 2025Review
- Engineering ofEngineering microbiology · 2023Review
- Big data mining, rational modification, and ancestral sequence reconstruction inferred multiple xylose isomerases for biorefinery.Science advances · 2023Article
- PHB production from cellobiose with Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- A novel D-xylose isomerase from the gut of the wood feeding beetle Odontotaenius disjunctus efficiently expressed in Saccharomyces cerevisiae.Scientific reports · 2021Article
- Novel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinantBiotechnology for biofuels · 2020Article
- Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation inBiotechnology for biofuels · 2019Article
- Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts.Frontiers in microbiology · 2018Review
- Screening and evolution of a novel protist xylose isomerase from the termiteBiotechnology for biofuels · 2017Article
- Xylose Fermentation by Saccharomyces cerevisiae: Challenges and Prospects.International journal of molecular sciences · 2016Review
- Bacterial xylose isomerases from the mammal gut Bacteroidetes cluster function in Saccharomyces cerevisiae for effective xylose fermentation.Microbial cell factories · 2015Article
- Metabolic engineering of yeasts by heterologous enzyme production for degradation of cellulose and hemicellulose from biomass: a perspective.Frontiers in microbiology · 2014Review
- Leveraging transcription factors to speed cellobiose fermentation by Saccharomyces cerevisiae.Biotechnology for biofuels · 2014Article
- Systematic and evolutionary engineering of a xylose isomerase-based pathway in Saccharomyces cerevisiae for efficient conversion yields.Biotechnology for biofuels · 2014Article
- Overcoming inefficient cellobiose fermentation by cellobiose phosphorylase in the presence of xylose.Biotechnology for biofuels · 2014Article
- Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins.Microbial cell factories · 2013Article
- Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24.Biotechnology for biofuels · 2013Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have integrated and coordinately expressed in Saccharomyces cerevisiae a xylose isomerase and cellobiose phosphorylase from Ruminococcus flavefaciens that enables fermentation of glucose, xylose, and cellobiose under completely anaerobic conditions. The native xylose isomerase was active in cell-free extracts from yeast transformants containing a single integrated copy of the gene. We improved the activity of the enzyme and its affinity for xylose by modifications to the 5'-end of the gene, site-directed mutagenesis, and codon optimization. The improved enzyme, designated RfCO*, demonstrated a 4.8-fold increase in activity compared to the native xylose isomerase, with a K(m) for xylose of 66.7 mM and a specific activity of 1.41 μmol/min/mg. In comparison, the native xylose isomerase was found to have a K(m) for xylose of 117.1 mM and a specific activity of 0.29 μmol/min/mg. The coordinate over-expression of RfCO* along with cellobiose phosphorylase, cellobiose transporters, the endogenous genes GAL2 and XKS1, and disruption of the native PHO13 and GRE3 genes allowed the fermentation of glucose, xylose, and cellobiose under completely anaerobic conditions. Interestingly, this strain was unable to utilize xylose or cellobiose as a sole carbon source for growth under anaerobic conditions, thus minimizing yield loss to biomass formation and maximizing ethanol yield during their fermentation.
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