ArticleThe Journal of biological chemistry2012
Polycomb repressive complex 1 (PRC1) disassembles RNA polymerase II preinitiation complexes.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 76 citations in OpenAlex.
- Hierarchical chromatin polyvalency governs robust gene regulation and organogenesis.bioRxiv : the preprint server for biology · 2026Article
- The Polycomb system sustains promoters in a deep OFF state by limiting pre-initiation complex formation to counteract transcription.Nature cell biology · 2024Article
- Synthetic Reader-Actuators Targeted to Polycomb-Silenced Genes Block Triple-Negative Breast Cancer Proliferation and Invasion.GEN biotechnology · 2023Article
- Deletion of taf1 and taf5 in zebrafish capitulate cardiac and craniofacial abnormalities associated with TAFopathies through perturbations in metabolism.Biology open · 2023Article
- Therapy Resistance and Disease Progression in CML: Mechanistic Links and Therapeutic Strategies.Current hematologic malignancy reports · 2022Review
- Review
- PRC1-independent binding and activity of RYBP on the KSHV genome during de novo infection.PLoS pathogens · 2022Article
- Histone modifiers at the crossroads of oncolytic and oncogenic viruses.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Review
- ProbC: joint modeling of epigenome and transcriptome effects in 3D genome.BMC genomics · 2022Article
- Adipocyte-mediated epigenomic instability in human T-ALL cells is cytotoxic and phenocopied by epigenetic-modifying drugs.Frontiers in cell and developmental biology · 2022Article
- Epigenetics of Cutaneous Sarcoma.International journal of molecular sciences · 2021Review
- The molecular principles of gene regulation by Polycomb repressive complexes.Nature reviews. Molecular cell biology · 2021Review
- PRC1 drives Polycomb-mediated gene repression by controlling transcription initiation and burst frequency.Nature structural & molecular biology · 2021Article
- Review
- Dynamics of H3K27me3 Modification on Plant Adaptation to Environmental Cues.Plants (Basel, Switzerland) · 2021Review
- BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome.Genes & development · 2021Article
- Genome-wide analysis of H3K4me3 and H3K27me3 modifications due to Lr28 for leaf rust resistance in bread wheat (Triticum aestivum).Plant molecular biology · 2020Article
- Components from the Human c-myb Transcriptional Regulation System Reactivate Epigenetically Repressed Transgenes.International journal of molecular sciences · 2020Article
- A functional genetic screen identifies the Mediator complex as essential for SSX2-induced senescence.Cell death & disease · 2019Article
- Lysine-specific post-translational modifications of proteins in the life cycle of viruses.Cell cycle (Georgetown, Tex.) · 2019Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Despite the important role of Polycomb in genome-wide silencing, little is known of the specific biochemical mechanism by which it inactivates transcription. Here we address how recombinant Polycomb repressive complex 1 (PRC1) inhibits activated RNA polymerase II preinitiation complex (PIC) assembly using immobilized H3K27-methylated chromatin templates in vitro. Recombinant PRC1 inhibited transcription, but had little effect on binding of the activator as reported previously. In contrast, Mediator and the general transcription factors were blocked during assembly or dissociated from preassembled PICs. Importantly, among the PIC components, Tata Binding Protein (TBP) was the most resistant to eviction by PRC1. Immobilized template experiments using purified PRC1, transcription factor II D (TFIID), and Mediator indicate that PRC1 blocks the recruitment of Mediator, but not TFIID. We conclude that PRC1 functions to block or dissociate PICs by interfering with Mediator, but leaves TBP and perhaps TFIID intact, highlighting a specific mechanism for PRC1 transcriptional silencing. Analysis of published genome-wide datasets from mouse embryonic stem cells revealed that the Ring1b subunit of PRC1 and TBP co-enrich at developmental genes. Further, genes enriched for Ring1b and TBP are expressed at significantly lower levels than those enriched for Mediator, TBP, and Ring1b. Collectively, the data are consistent with a model in which PRC1 and TFIID could co-occupy genes poised for activation during development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.