ArticleHistochemistry and cell biology2013
Single-molecule coordinate-based analysis of the morphology of HIV-1 assembly sites with near-molecular spatial resolution.
Article in Histochemistry and cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 65 citations in OpenAlex.
- Provirus activation plus CD59 blockage triggers antibody-dependent complement-mediated lysis of latently HIV-1-infected cells.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- The ARVC-5-associated protein TMEM43 controls mitochondrial energy metabolism by stabilising ER-mitochondrial contact sites.Cellular and molecular life sciences : CMLS · 2025Article
- PIPScience advances · 2025Article
- Visualizing HIV-1 Assembly at the T-Cell Plasma Membrane Using Single-Molecule Localization Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- HIV-1 Gag targeting to the plasma membrane reorganizes sphingomyelin-rich and cholesterol-rich lipid domains.Nature communications · 2023Article
- Mapping molecular complexes with super-resolution microscopy and single-particle analysis.Open biology · 2022Review
- Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design.Frontiers in bioengineering and biotechnology · 2021Review
- Raw Data to Results: A Hands-On Introduction and Overview of Computational Analysis for Single-Molecule Localization Microscopy.Frontiers in bioinformatics · 2021Article
- A Review of Super-Resolution Single-Molecule Localization Microscopy Cluster Analysis and Quantification Methods.Patterns (New York, N.Y.) · 2020Review
- Single-Virus Tracking: From Imaging Methodologies to Virological Applications.Chemical reviews · 2020Review
- Monitoring HIV-1 Assembly in Living Cells: Insights from Dynamic and Single Molecule Microscopy.Viruses · 2019Review
- Super-resolution fluorescence microscopy studies of human immunodeficiency virus.Retrovirology · 2018Review
- Clus-DoC: a combined cluster detection and colocalization analysis for single-molecule localization microscopy data.Molecular biology of the cell · 2016Article
- On characterizing protein spatial clusters with correlation approaches.Scientific reports · 2016Article
- Blockage of CD59 Function Restores Activities of Neutralizing and Nonneutralizing Antibodies in Triggering Antibody-Dependent Complement-Mediated Lysis of HIV-1 Virions and Provirus-Activated Latently Infected Cells.Journal of virology · 2015Article
- Artifacts in single-molecule localization microscopy.Histochemistry and cell biology · 2015Article
- Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites.PLoS pathogens · 2015Article
- Structural analysis of herpes simplex virus by optical super-resolution imaging.Nature communications · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We apply single-molecule super-resolution microscopy and coordinate-based cluster analysis to extract information on the distribution and on the morphology and size of clusters of the human immunodeficiency virus (HIV-1) Gag polyprotein in fixed cells. Three different patterns of Gag distribution could be distinguished. A major type of assembly observed was in accordance with previous electron microscopy analyses revealing ~140 nm-sized HIV-1 buds at the plasma membrane of virus-producing cells. The distribution of Gag molecules in the 2D projection at these sites was consistent with a semi-spherical 3D assembly. We compared different methods of cluster analysis and demonstrated that we can reliably distinguish different distribution patterns of the Gag polyprotein. These methods were applied to extract information on the properties of the different Gag clusters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.