ArticleMethods in enzymology2012
Experimental analysis of the mechanism of chromatin remodeling by RNA polymerase II.
Article in Methods in enzymology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Histone tetrasome dynamics affects chromatin transcription.Nucleic acids research · 2025Article
- Human PARP1 Facilitates Transcription through a Nucleosome and Histone Displacement by Pol II In Vitro.International journal of molecular sciences · 2022Article
- NaMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2022Article
- Time-resolved analysis of transcription through chromatin.Methods (San Diego, Calif.) · 2019Article
- Mechanism of RNA polymerase II stalling by DNA alkylation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Preparation of mononucleosomal templates for analysis of transcription with RNA polymerase using spFRET.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Preparation and analysis of positioned mononucleosomes.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Experimental analysis of hFACT action during Pol II transcription in vitro.Methods in molecular biology (Clifton, N.J.) · 2015Article
- A novel, non-radioactive eukaryotic in vitro transcription assay for sensitive quantification of RNA polymerase II activity.BMC molecular biology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The vital process of transcription by RNA polymerase II (Pol II) occurs in chromatin environment in eukaryotic cells; in fact, moderately transcribed genes retain nucleosomal structure. Recent studies suggest that chromatin structure presents a strong barrier for transcribing Pol II in vitro, and that DNA-histone interactions are only partially and transiently disrupted during transcript elongation on moderately active genes. Furthermore, elongating Pol II complex is one of the major targets during gene regulation. Below, we describe a highly purified, defined experimental system that recapitulates many important properties of transcribed chromatin in vitro and allows detailed analysis of the underlying mechanisms.
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Registered trials
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