ArticleMethods in enzymology2012
Enzymatic analysis of Tet proteins: key enzymes in the metabolism of DNA methylation.
Article in Methods in enzymology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- 5-Hydroxymethylcytosine: Far Beyond the Intermediate of DNA Demethylation.International journal of molecular sciences · 2024Review
- Development of a rapid mass spectrometric method for the analysis of ten-eleven translocation enzymes.Methods in enzymology · 2024Article
- Hydroxymethylation and tumors: can 5-hydroxymethylation be used as a marker for tumor diagnosis and treatment?Human genomics · 2020Review
- Tet2-mediated epigenetic drive for astrocyte differentiation from embryonic neural stem cells.Cell death discovery · 2020Article
- Who's your daddy? Behavioral and epigenetic consequences of paternal drug exposure.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2019Review
- Modulation of mitochondrial DNA copy number in a model of glioblastoma induces changes to DNA methylation and gene expression of the nuclear genome in tumours.Epigenetics & chromatin · 2018Article
- Eight Kinetically Stable but Thermodynamically Activated Molecules that Power Cell Metabolism.Chemical reviews · 2018Review
- A rapid mass spectrometric method for the measurement of catalytic activity of ten-eleven translocation enzymes.Analytical biochemistry · 2017Article
- Understanding the epigenetic basis of sex differences in depression.Journal of neuroscience research · 2017Review
- Tissue-specific methylation profile in obese patients with type 2 diabetes before and after Roux-en-Y gastric bypass.Diabetology & metabolic syndrome · 2017Review
- Cofactors As Metabolic Sensors Driving Cell Adaptation in Physiology and Disease.Frontiers in endocrinology · 2017Review
- TET1 knockdown inhibits the odontogenic differentiation potential of human dental pulp cells.International journal of oral science · 2016Article
- A Fluorescence Polarization Biophysical Assay for the Naegleria DNA Hydroxylase Tet1.ACS medicinal chemistry letters · 2016Article
- Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity.Methods in enzymology · 2016Article
- Measurement of Postreplicative DNA Metabolism and Damage in the Rodent Brain.Chemical research in toxicology · 2015Article
- Quantification of 5-methylcytosine, 5-hydroxymethylcytosine and 5-carboxylcytosine from the blood of cancer patients by an enzyme-based immunoassay.Analytica chimica acta · 2014Article
- Nanopores discriminate among five C5-cytosine variants in DNA.Journal of the American Chemical Society · 2014Article
- Ikbkap/Elp1 deficiency causes male infertility by disrupting meiotic progression.PLoS genetics · 2013Article
- Sex, stress, and epigenetics: regulation of behavior in animal models of mood disorders.Biology of sex differences · 2013Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
One of the most exciting recent advances in the epigenetic field is the discovery that 5-methylcytosine (5mC) in DNA can be iteratively oxidized by a family of proteins known as Tet proteins to generate 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). These 5mC derivatives can be further processed by thymine-DNA glycosylase (TDG) followed by base excision repair or by replication-dependent dilution leading to DNA demethylation. Given the similarity between 5mC and its oxidation derivatives, many of the conventional techniques used for 5mC analysis cannot distinguish between 5mC and 5hmC/5fC/5caC. Here, we describe 2D-TLC and mass spectrometry methods that we have successfully used in differentiating 5mC from its oxidative derivatives as well as in characterizing the enzymatic activity of Tet proteins both in vitro and in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.