Evidence map›Paper›PMID 22909954›Full record

ArticleChannels (Austin, Tex.)

Selective fluorophore-assisted light inactivation of voltage-gated calcium channels.

Evgeny Kobrinsky, Jung-Ha Lee, Nikolai M Soldatov

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Channels (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 2 countries.

Evgeny KobrinskyNational Institute on Aging, National Institutes of Health, Baltimore, MD, USA.
Jung-Ha Lee
Nikolai M Soldatov
Institute on Aging · USNational Institute on Aging · USSogang University · KR

Funding

MOLECULAR CORRELATES OF HUMAN CALCIUM CHANNEL REGULATIONZ01AG000294 · NIA · NATIONAL INSTITUTE ON AGING · PI SOLDATOV, NIKOLAI · 2000 to 2008
$2.3M
PHYSICIAN SCIENTIST PROGRAM AWARDK12AG000294 · NIA · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI LIPSITZ, LEWIS A · 1985 to 2003
$1.8M
Intramural NIH HHS Z01 AG000294NIA NIH HHS K12 AG000294
6 · The paper itself

Abstract

Fluorophore-assisted light inactivation (FALI) is an investigative tool to inactivate fluorescently labeled proteins by a mechanism of in situ photodestruction. We found that Ca(v)1.2 (L-type) and Ca(v)3.1 (T-type) calcium channels, labeled by genetic fusion with GFP derivatives, show differential sensitivity to FALI. Specifically, FALI silences Ca(v)1.2 calcium channels containing EYFP-labeled α(1C)subunits but does not affect the EYFP-α(1G) Ca(v)3.1 calcium channels or Ca(v)1.2 channels containing EYFP-labeled β subunits. Our findings limit the applicability of acceptor photobleaching for the measurements of FRET but open an opportunity to combine the fluorescent imaging of the live cell expressing labeled calcium channels with selective functional inactivation of their specific subsets.

Indexed as

Chromophore-Assisted Light InactivationAnimalsCalcium Channels, L-TypeCalcium Channels, T-TypeChlorocebus aethiopsCOS CellsFluorescence Resonance Energy TransferGreen Fluorescent ProteinsIon Channel GatingMicroscopy, FluorescencePhotobleachingRecombinant Fusion ProteinsUltraviolet RaysCalcium Channels, L-TypeCalcium Channels, T-TypeGreen Fluorescent ProteinsRecombinant Fusion Proteins

Identifiers

PMID22909954
PMCPMC3431587
OpenAlexW2061521379

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.