ArticleThe Journal of cell biology2012
A novel split kinesin assay identifies motor proteins that interact with distinct vesicle populations.
Article in The Journal of cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 77 citations in OpenAlex.
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- Kinesin Regulation in the Proximal Axon is Essential for Dendrite-selective Transport.Molecular biology of the cell · 2024Article
- Polarized transport requires AP-1-mediated recruitment of KIF13A and KIF13B at the trans-Golgi.Molecular biology of the cell · 2024Article
- FACI is a novel clathrin adaptor protein 2-binding protein that facilitates low-density lipoprotein endocytosis.Cell & bioscience · 2023Article
- Axonal transport during injury on a theoretical axon.Frontiers in cellular neuroscience · 2023Article
- KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC.Molecular biology of the cell · 2022Article
- Propofol attenuates kinesin-mediated axonal vesicle transport and fusion.Molecular biology of the cell · 2022Article
- Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation.Life science alliance · 2022Article
- ER and Golgi trafficking in axons, dendrites, and glial processes.Current opinion in cell biology · 2022Review
- Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.EMBO reports · 2022Article
- Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons.Methods in molecular biology (Clifton, N.J.) · 2022Article
- KIF13B-mediated VEGFR2 trafficking is essential for vascular leakage and metastasis in vivo.Life science alliance · 2022Article
- NgCAM and VAMP2 reveal that direct delivery and dendritic degradation maintain axonal polarity.Molecular biology of the cell · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Identifying the kinesin motors that interact with different vesicle populations is a longstanding and challenging problem with implications for many aspects of cell biology. Here we introduce a new live-cell assay to assess kinesin-vesicle interactions and use it to identify kinesins that bind to vesicles undergoing dendrite-selective transport in cultured hippocampal neurons. We prepared a library of "split kinesins," comprising an axon-selective kinesin motor domain and a series of kinesin tail domains that can attach to their native vesicles; when the split kinesins were assembled by chemical dimerization, bound vesicles were misdirected into the axon. This method provided highly specific results, showing that three Kinesin-3 family members-KIF1A, KIF13A, and KIF13B-interacted with dendritic vesicle populations. This experimental paradigm allows a systematic approach to evaluate motor-vesicle interactions in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.