ArticleMethods in molecular biology (Clifton, N.J.)2012
Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenome.Nature cell biology · 2022Article
- Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct.Molecules and cells · 2015Article
- Longitudinal epigenetic and gene expression profiles analyzed by three-component analysis reveal down-regulation of genes involved in protein translation in human aging.Nucleic acids research · 2015Article
- De novo DNA methylation in the male germ line occurs by default but is excluded at sites of H3K4 methylation.Cell reports · 2013Article
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Authors and funding
2 authors.
Funding
Abstract
Imprinted genes are marked by parental allele specific DNA methylation and histone modifications which regulate their monoallelic expression. Chromatin immunoprecipitation (ChIP) is the technique of choice to characterize the histones associated with either maternal or paternal chromosomes. To study allele-specific chromatin composition at imprinted regions, the method has to be efficient to work on limiting amount of starting material, and specific enough to recognize one of the parental alleles. We optimized the commonly used ChIP technique for efficient recovery of one parental allele from small number of cells. We provide examples to show that this ChIP protocol can specifically distinguish between parental alleles in mouse embryo fibroblasts carrying maternal and paternal duplication of mouse distal Chr7 and also in normal mouse embryo fibroblasts carrying single nucleotide polymorphism at imprinted regions.
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