ArticlePloS one2012
Environmental factors influencing gene transfer agent (GTA) mediated transduction in the subtropical ocean.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- The mechanism of biofilm degradation by a detachable tailspike of gene transfer agents.bioRxiv : the preprint server for biology · 2026Article
- With a pinch of salt: metagenomic insights into Namib Desert salt pan microbial mats and halites reveal functionally adapted and competitive communities.Applied and environmental microbiology · 2023Article
- Article
- Gene Transfer Agents in Symbiotic Microbes.Results and problems in cell differentiation · 2020Review
- Insights into origin and evolution of α-proteobacterial gene transfer agents.Virus evolution · 2017Article
- Microbial Surface Colonization and Biofilm Development in Marine Environments.Microbiology and molecular biology reviews : MMBR · 2016Review
- Molecular mechanisms underlying the emergence of bacterial pathogens: an ecological perspective.Molecular plant pathology · 2016Review
- Gene Flow Across Genus Barriers - Conjugation of Dinoroseobacter shibae's 191-kb Killer Plasmid into Phaeobacter inhibens and AHL-mediated Expression of Type IV Secretion Systems.Frontiers in microbiology · 2016Article
- "You produce while I clean up", a strategy revealed by exoproteomics during Synechococcus-Roseobacter interactions.Proteomics · 2015Article
- Coral transplantation triggers shift in microbiome and promotion of coral disease associated potential pathogens.Scientific reports · 2015Article
- Noncanonical cell-to-cell DNA transfer in Thermus spp. is insensitive to argonaute-mediated interference.Journal of bacteriology · 2015Article
- Bioinformatics analysis of bacterial annexins--putative ancestral relatives of eukaryotic annexins.PloS one · 2014Article
- Phosphate concentration and the putative sensor kinase protein CckA modulate cell lysis and release of the Rhodobacter capsulatus gene transfer agent.Journal of bacteriology · 2013Article
- Coral-associated micro-organisms and their roles in promoting coral health and thwarting diseases.Proceedings. Biological sciences · 2013Review
- Environmental factors influencing gene transfer agent (GTA) mediated transduction in the subtropical ocean.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial genomic sequence analyses have indicated widespread horizontal gene transfer (HGT). However, an adequate mechanism accounting for the ubiquity of HGT has been lacking. Recently, high frequencies of interspecific gene transfer have been documented, catalyzed by Gene Transfer Agents (GTAs) of marine α-Proteobacteria. It has been proposed that the presence of bacterial genes in highly purified viral metagenomes may be due to GTAs. However, factors influencing GTA-mediated gene transfer in the environment have not yet been determined. Several genomically sequenced strains containing complete GTA sequences similar to Rhodobacter capsulatus (RcGTA, type strain) were screened to ascertain if they produced putative GTAs, and at what abundance. Five of nine marine strains screened to date spontaneously produced virus-like particles (VLP's) in stationary phase. Three of these strains have demonstrated gene transfer activity, two of which were documented by this lab. These two strains Roseovarius nubinhibens ISM and Nitratireductor 44B9s, were utilized to produce GTAs designated RnGTA and NrGTA and gene transfer activity was verified in culture. Cell-free preparations of purified RnGTA and NrGTA particles from marked donor strains were incubated with natural microbial assemblages to determine the level of GTA-mediated gene transfer. In conjunction, several ambient environmental parameters were measured including lysogeny indicated by prophage induction. GTA production in culture systems indicated that approximately half of the strains produced GTA-like particles and maximal GTA counts ranged from 10-30% of host abundance. Modeling of GTA-mediated gene transfer frequencies in natural samples, along with other measured environmental variables, indicated a strong relationship between GTA mediated gene transfer and the combined factors of salinity, multiplicity of infection (MOI) and ambient bacterial abundance. These results indicate that GTA-mediated HGT in the marine environment with the strains examined is favored during times of elevated bacterial and GTA abundance as well as in areas of higher salinity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.