ArticlePloS one2012
Identification of protective pneumococcal T(H)17 antigens from the soluble fraction of a killed whole cell vaccine.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
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- Generation of protective pneumococcal-specific nasal resident memory CD4Mucosal immunology · 2020Article
- Vitamin D Induces Differential Effects on Inflammatory Responses During Bacterial and/or Viral Stimulation of Human Peripheral Blood Mononuclear Cells.Frontiers in immunology · 2020Article
- Next-Generation Whole-Cell Pneumococcal Vaccine.Vaccines · 2019Review
- Serotype-Independent Protection Against Invasive Pneumococcal Infections Conferred by Live Vaccine WithFrontiers in immunology · 2019Article
- Screening for Th17-Dependent Pneumococcal Vaccine Antigens: Comparison of Murine and Human Cellular Immune Responses.Infection and immunity · 2018Article
- Recognition of conserved antigens by Th17 cells provides broad protection against pulmonaryProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Identification of SP1683 as a pneumococcal protein that is protective against nasopharyngeal colonization.Human vaccines & immunotherapeutics · 2018Article
- Immunodominance in T cell responses elicited against different domains of detoxified pneumolysin PlyD1.PloS one · 2018Article
- Recent approaches in whole cell pneumococcal vaccine development: a review study.Iranian journal of microbiology · 2017Review
- Interluekin-17A (IL17A).Gene · 2017Review
- Protection against Streptococcus pneumoniae lung infection after nasopharyngeal colonization requires both humoral and cellular immune responses.Mucosal immunology · 2015Article
- Reduced IL-17A Secretion Is Associated with High Levels of Pneumococcal Nasopharyngeal Carriage in Fijian Children.PloS one · 2015Article
- Effect of nonheme iron-containing ferritin Dpr in the stress response and virulence of pneumococci.Infection and immunity · 2014Article
- Activation of memory Th17 cells by domain 4 pneumolysin in human nasopharynx-associated lymphoid tissue and its association with pneumococcal carriage.Mucosal immunology · 2014Article
- Article
- The host immune dynamics of pneumococcal colonization: implications for novel vaccine development.Human vaccines & immunotherapeutics · 2014Review
- Multiple antigen-presenting system (MAPS) to induce comprehensive B- and T-cell immunity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mucosal or parenteral immunization with a killed unencapsulated pneumococcal whole cell antigen (WCA) with an adjuvant protects mice from colonization by a T(H)17 CD4+ cell-mediated mechanism. Using preparative SDS gels, we separated the soluble proteins that compose the WCA in order to identify fractions that were immunogenic and protective. We screened these fractions for their ability to stimulate IL-17A secretion from splenocytes obtained from mice immunized with WCA and adjuvant. We identified 12 proteins within the stimulatory fractions by mass spectrometry; these proteins were then cloned, recombinantly expressed and purified using an Escherichia coli expression system. The ability of these proteins to induce IL-17A secretion was then evaluated by stimulation of mouse splenocytes. Of the four most stimulatory proteins, three were protective in a mouse pneumococcal serotype 6B colonization model. This work thus describes a method for identifying immunogenic proteins from the soluble fraction of pneumococcus and shows that several of the proteins identified protect mice from colonization when used as mucosal vaccines. We propose that, by providing protection against pneumococcal colonization, one or more of these proteins may serve as components of a multivalent pneumococcal vaccine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.